| 姜黄素对急性肺损伤大鼠的保护作用及分子机制研究 |
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| 引用本文： | 刘玉斌,罗百灵,谢静,张丽. 姜黄素对急性肺损伤大鼠的保护作用及分子机制研究[J]. 中国热带医学, 2011, 11(5): 586-588 |
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| 作者姓名： | 刘玉斌 罗百灵 谢静 张丽 |
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| 作者单位： | 1. 中南大学湘雅医院呼吸内科,湖南,长沙,410008;湘南学院附属医院呼吸内科,湖南,郴州,423000
2. 中南大学湘雅医院呼吸内科,湖南,长沙,410008 |
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| 摘 要： | 目的探讨血红素加氧酶（HO-1）在姜黄素减轻脂多糖（LPS）所致急性肺损伤（ALI）中的作用。方法将大鼠随机分为5组：正常组、ALI模型、LPS＋姜黄素组、LPS＋姜黄素＋氯血红素（Hm）组以及LPS＋姜黄素＋锌原卟啉（ZnPP）组。给药后进行肺组织的形态学观察,并测定丙二醛（MDA）的含量以及HO-1的活性;RT-PCR和Western blot检测HO-1 mRNA和蛋白的表达情况。结果用LPS成功复制出ALI模型,同时,肺组织中MDA含量、HO-1蛋白活性、HO-1 mRNA以及蛋白水平均高于阴性对照组（P均〈0.05）;姜黄素＋LPS组、姜黄素＋LPS＋Hm组肺组织损伤程度、MDA含量低于LPS模型组,但HO-1活性、mRNA和蛋白水平高于相应LPS组（P均〈0.05）。LPS＋姜黄素＋ZnPP组肺损伤程度、MDA含量高于LPS组,而HO-1活性、mRNA和蛋白水平低于LPS组（P〈0.05）。结论姜黄素对ALI的保护作用可能通过增强HO-1活性、上调mRNA和蛋白表达水平而实现的。
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| 关 键 词： | 急性肺损伤 姜黄素 血红素加氧酶 |
Molecular mechanism and protective effect of curcumin on acute lung injury rats |
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| LIU Yu-bin,LUO Bai-ling,XIE Jing,et al.. Molecular mechanism and protective effect of curcumin on acute lung injury rats[J]. China Tropical Medicine, 2011, 11(5): 586-588 |
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| Authors: | LIU Yu-bin LUO Bai-ling XIE Jing et al. |
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| Affiliation: | LIU Yu-bin,LUO Bai-ling,XIE Jing,et al.(Xiangya Medical College of Zhongnan University,Changsha 410008,Hunan,P.R.China) |
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| Abstract: | Aim To study the role of heme oxygenase(HO)-1 in the curcumin for attenuation of acute lung injury(ALI) induced by lipopolysaccharide curcumin(LPSC).Methods Experimental rats were randomly divided into five groups:control group,LPSC group,curcumin+ LPSC group,LPSC +curcumin+hemin gourp and LPSC +curcumin+zinc protoporphyrin gourp.The structure of the lung,MDA content,HO-1 activity,mRNA of HO-1 and protein expression were detemined.Results The ALI model was successfully constructed by LPSC,and the levels of ... |
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| Keywords: | Acute lung jury Curcumin Heme oxygenase1 |
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