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| 梅毒螺旋体0751基因的克隆、表达及鉴定 | |
| 引用本文： | 马强,耿焱,黄茂梁,吴英松,李明.梅毒螺旋体0751基因的克隆、表达及鉴定[J].热带医学杂志,2011,11(6):637-638,649. |
| 作者姓名： | 马强 耿焱 黄茂梁 吴英松 李明 |
| 作者单位： | 1. 南方医科大学生物技术学院抗体工程研究所,广东广州,510515 2. 解放军南宁303医院内科,广西南宁,530021 3. 武警福州总医院内科,福建福州,350003 |
| 基金项目： | 国家科技重大专项(2009ZX10004-706) |
| 摘 要： | 目的 构建梅毒螺旋体0751基因的原核表达质粒,诱导重组蛋白表达及进行免疫学鉴定.方法 以梅毒螺旋体基因组DNA为模板,PCR扩增梅毒螺旋体0751基因,构建其重组原核表达载体,并采用Western-blot方法初步鉴定该蛋白的特异性.结果 成功扩增出梅毒螺旋体0751基因,该基因在大肠杆菌表达系统中得到表达,并且特异性识别梅毒患者血清.结论 在大肠杆菌中成功表达梅毒螺旋体0751基因,为梅毒螺旋体的诊断提供了新的特异性抗原. |
| 关 键 词： | 梅毒螺旋体 0751基因 蛋白表达 |
Cloning,expression and identification of the 0751 gene from Treponema pallidun |
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| MA Qiang ,GENG Yan ,HUANG Mao-liang ,WU Ying-song ,LI Ming.Cloning,expression and identification of the 0751 gene from Treponema pallidun[J].Journal Of Tropical Medicine,2011,11(6):637-638,649. | |
| Authors: | MA Qiang GENG Yan HUANG Mao-liang WU Ying-song LI Ming |
| Institution: | MA Qiang 1,GENG Yan 2,HUANG Mao-liang 3,WU Ying-song 1,LI Ming 1(1.The Institute of Antibody Engineering,School of Biotechnology,Southern Medical University,Guangdong,Guangzhou 510515,2.Departments of Digestive Diseases,the No.303 Hospital of PLA,Guangxi,Nanning 530021,3.Departments of Digestive Diseases,the Fuzhou Hospital of Armed Police,Fujian,Fuzhou 350003,China) |
| Abstract: | Objective The aim of the study was to clone,express and identify of 0751 gene from Treponema pallidun.Methods 0751 gene was amplified by the PCR from the genomic DNA of Treponema pallidun and cloned into PET-28a(+) vector.After induction by IPTG,the 0751 protein was identified by Western-blot.Results The 0751 gene(552bp)was amplified and the recombinant protein was expressed successfully.Western-blot showed that recombinant 0751 could be identified specifically by Treponema pallidun infected serum.Conclusio... |
| Keywords: | Treponema pallidun 0751 gene protein expression |
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