益气活血中药复方对CVB3病毒性心肌炎心肌细胞actin、Cx43表达及GJIC功能的影响

目的观察益气活血中药复方对柯萨奇病毒B组3型(Coxsackie virus B3,CVB3)病毒性心肌炎心肌细胞间缝隙连接结构及通讯功能的影响。方法以免疫组化法检测正常组、病毒组、益气活血中药复方组心肌细胞肌动蛋白(actin)及连接蛋白(connexin43,Cx43)表达;,并以激光扫描共聚焦显微镜法检测各组心肌细胞荧光漂白恢复率。结果益气活血中药复方能上调CVB3病毒性心肌炎心肌细胞actin及Cx43表达,能显著改善细胞间缝隙连接通讯(GJIC)功能。结论益气活血中药复方具有对抗CVB3致心肌细胞骨架蛋白损伤,修复CVB3病毒性心肌炎心肌细胞间缝隙连接通道结构,改善CVB3攻击后心肌细胞GJIC功能的作用。

Effects of Chinese Herbal Compound for Supplementing Qi and Activating Blood Circulation on Actin,Cx43 Expressions and Gap Junctional Intercellular Communication Functions of Myocardial Cells in Patients with Coxsackie Virus B 3 Viral Myocarditis

Objective To observe the effect of Chinese herbal compound for supplementing qi and activating blood circulation（CHC） on the gap junctional intercellular communication（GJIC） function of myocardial cells in patients with Coxsackie virus B 3（CVB3） viral myocarditis.Methods Expressions of actin and connexin43（Cx43） in myocardial cells of patients arranged in three groups（the normal control group,the viral infected group and the CHC treated group） were detected by immunohistochemical method;the fluorescence photobleaching recovery rate of cells was detected by laser scanning confocal microscope.Results As compared with the viral infected group,the expressions of actin and Cx43 were increased and the GJIC function was improved in the CHC treated group.Conclusion CHC could antagonize viral injury on skeleton protein,and repair the structure of gap junction channel to improve the GJIC function of myocardial cells after being attacked by CVB3.