| HER-2癌基因反义寡脱氧核苷酸对乳腺癌细胞放射敏感性的影响 |
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| 引用本文： | 谢娟,蔡丽娜,周军. HER-2癌基因反义寡脱氧核苷酸对乳腺癌细胞放射敏感性的影响[J]. 贵州医药, 2006, 30(12): 1059-1062 |
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| 作者姓名： | 谢娟 蔡丽娜 周军 |
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| 作者单位： | 贵州省人民医院药剂科,贵阳,550002;贵州省人民医院药剂科,贵阳,550002;贵州省人民医院药剂科,贵阳,550002 |
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| 摘 要： | 目的探讨癌基因HER-2反义寡核苷酸联合放疗对乳腺癌细胞的影响。方法实验分组如下:HER-2反义寡脱氧核苷酸组、HER-2正义寡脱氧核苷酸组、叶酸对照组和空白对照组。用RT-PCR方法、MTT法及平板克隆形成实验检测乳腺癌细胞SK-Br-3受药物处理后,经60Coγ射线照射,HER-2表达水平、细胞存活分数和集落形成率的改变。结果经HER-2反义寡核苷酸-叶酸处理的细胞,对60Coγ射线的放射敏感性增加,细胞的HER-2癌基因表达水平降低,存活分数及集落形成率与正义寡核苷酸、叶酸和空白对照组比较明显降低(P<0.05),而正义寡核苷酸、叶酸与空白对照组相比无明显差异(P>0.05)。结论HER-2反义寡核苷酸抑制癌基因表达的作用增加乳腺癌细胞SK-Br-3对放疗的敏感性。
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| 关 键 词： | HER-2反义寡脱氧核苷酸 叶酸 乳腺癌 放射敏感性 |
| 文章编号： | 1000-744X（2006）12-1059-04 |
Effect of HER-2 oncogene antisense oligodeoxynucleotides on radiosensityivity of breast cancer cell line |
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| Xie Juan,Cai Lina,Zhou Jun. Effect of HER-2 oncogene antisense oligodeoxynucleotides on radiosensityivity of breast cancer cell line[J]. Guizhou Medical Journal, 2006, 30(12): 1059-1062 |
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| Authors: | Xie Juan Cai Lina Zhou Jun |
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| Abstract: | Objective To explore the effect of HER-2 oncogene antisense oligodeoxynucleotides on radiosensitivity of breast cancer cell line. Methods To set up HER-2 antisense oligodeoxynucleotides (ASODN) experimental group, sense oligodeoxynucleotides (SODN) group, folate acid (FA) group and 60Co irradiation control group. The expression of HER-2 was detected by means of RT-PCR. Cellular response to irradiation was evaluated by the colony forming test and MTT assay.Results HER-2 ASODN could suppress the expression of c-erbB2 which significantly increased the radiosensitivity of breast cancer cells (P<0.05), while folate acid group and the SODN group did not increase the radiosensitivity of SK-Br-3 cell line.Conclusion HER-2 antisense oligodeoxynucleotides sensitized the breast cancer cell SK-Br-3 to ionizing irradiation through decreasing the expression of HER-2. |
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| Keywords: | HER-2 antisense oligodeoxynucleotides Folate acid Breast cancer Radiosensitivity |
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