颞肌贴敷术联合自体骨髓干细胞动员治疗大鼠脑缺血

目的探讨颞肌贴敷术联合自体骨髓干细胞动员治疗脑缺血的疗效及机制。方法选取健康成年雄性SD大鼠，线栓法制作脑缺血模型，随机分为对照组、颞肌贴敷术组、自体骨髓干细胞动员组、颞肌贴敷术联合自体骨髓干细胞动员组。采用神经功能缺损评分检测大鼠神经系统功能，病理和免疫组织化学检测组织缺血、BrdU和Ⅷ因子抗原，TUNEL法检测细胞凋亡。结果颞肌贴敷术联合自体骨髓干细胞动员组神经功能缺损评分明显低于颞肌贴敷术组和自体骨髓干细胞动员组（P〈0．01），后两组神经功能缺损评分均明显低于对照组（P〈0．05）；组织病理显示颞肌贴敷术联合自体骨髓干细胞动员组缺血坏死面积、细胞凋亡数较颞肌贴敷术组和自体骨髓干细胞动员组明显减少（P〈0．05），后两组细胞凋亡数均明显低于对照组（P〈0．05）；颞肌贴敷术联合自体骨髓干细胞动员组BrdU阳性细胞数、微血管数较颞肌贴敷术组和自体骨髓干细胞动员组均明显增多（P〈0．05），后两组BrdU阳性细胞数、微血管数均较对照组显著增多（P〈0．05）。结论颞肌贴敷术联合白体干细胞动员可通过减少梗死面积，促进血管生成，改善微循环，减少缺血灶周围细胞凋亡，增进神经的再生与修复，改善脑功能。

Temporal muscle sticking combined with autologous bone marrow stem cell mobilization for the treatment of ischemic cerebrovascular diseases in rats

Objective To explore the effect and mechanism of treatment to the ischemic cerebrovascular diseases, treatment with temporal muscle sticking combined with autologous bone marrow stem cell mobilization. Methods Healthy adult male Sprague-Dawley rats, to set up animal models in rat with transient focal cerebral ischemia by intraluminal occlusion using monofilament, randomly divided into control group; temporal muscle sticking group ; autologous bone marrow stem cell mobilization group; temporal muscle sticking combined with autologous bone marrow stem cell mobilization group. Using neurological deficit scores detected the function of the nervous system in rats. Histopathology observation, immunohistochemistry detects BrdU and Factor Ⅷ antigen. Terminal deoxynucleotidyl Transferase Biotin- dUTP Nick End Labeling （ TUNEL ） detects apoptosis. Results Temporal muscle sticking combined with autologous bone marrow stem cell mobilization group neurological deficit score is significantly lower than temporal muscle sticking group and bone marrow stem cell mobilization group （ P 〈 0. 01 ）. The latter two groups neurological deficit score was significantly lower than control group （ P 〈 0. 05）. Histopathology showed that temporal muscle sticking combined with autologous bone marrow stem cell mobilization group necrosis is abatement,apoptosis is much less than temporal muscle sticking and autologous bone marrow stem cell mobilization（P 〈0. 05 ）. The latter two apoptosis is much less than control group;temporal muscle sticking combined with autologous bone marrow stem cell mobilization group BrdU positive cells ,angiogenesis is much more than temporal muscle sticking group and autologous bone marrow stem cell mobilization group （ P 〈 0. 05 ）. The latter two BrdU positive cells, angiogenesis is much more than control group （ P 〈 0.05 ）. Conclusions The temporal muscle sticking combined with autologous bone marrow stem cell mobilization can reduce the infarct area, promote microcirculation and angiogenesis, reduce apoptosis in focal ischemia area, promote nerve regeneration and repair, and then improve brain function.