代谢组学分析技术及代谢物鉴定

近年来,代谢组学技术在众多科学领域得到了广泛的应用。代谢组学研究的关键在于对大量小分子代谢产物进行快速、准确的分析鉴定,这在很大程度上依赖于相关技术的进步。磁共振、质谱、色谱以及毛细管电泳等技术的发展和联用,使得代谢组学的广泛应用成为可能。磁共振和质谱是代谢组学研究中最重要的两个技术平台,对代谢产物进行明确的鉴定是代谢组学研究的根本任务。本文就代谢组学相关分析技术及代谢物鉴定的研究进展作一综述。

Metabonomics analytical technologies and metabolite identification

In recent years, the metabonomics technologies have been widely used in numerous science fields. The rapid, accurate analysis and identification for a large number of small molecular metabolites is the key of metabonomics studies, which relies largely on the progress in relevant technologies. The development and combination of many technologies, such as nuclear magnetic resonance （NMR）, mass spectrometry（MS）, chromatography and capillary electrophoresis, make them possible for the wide application of metabonomics. NMR and MS are the most important technical platforms in metabonomics studies. The unambiguous identification of metabolites is the basic task of metabonomics studies. The research progress in metabonomics analytical technologies and metabolite identification is reviewed in this paper.