哮喘反应大鼠肺泡巨噬细胞产生IL-1增加

目的 :探讨哮喘反应大鼠肺泡巨噬细胞 (AM )产生白细胞介素 1(IL 1)的情况 ,明确IL 1在哮喘发病中的意义。方法 :用卵白蛋白致敏并激发大鼠 ,建立大鼠哮喘反应模型。致敏大鼠于激发后即刻、1h、4h和 16h行支气管肺泡灌洗 ,分离支气管肺泡灌洗液 (BALF)中AM ,在含或不含脂多糖 (LPS)条件下培养AM ,收集其上清液 ,用小鼠胸腺细胞增殖法测定AM上清液中IL 1活性。结果 :致敏大鼠在抗原激发后 1h和 4h的BALF中AM自发产生IL 1活性显著增加 (与对照组和即刻组相比 ,均P <0 .0 1) ;而LPS刺激后对照组和致敏大鼠抗原激发后不同时刻组间IL 1产生无明显差异 (均P >0 .0 5 )。结论 :哮喘反应大鼠AM在体内已被活化并释放IL 1增加。

Increased production of IL-1 of alveolar macrocytes in bronchial alveolar lavage fluid (BALF) of asthmatic rats

Objective:To evaluate production of IL-1 by alveolar macrocytes (AM) in BALF of asthmatic rats. Methods:Wister rats were sensitized by 1mg ovalbumin (OVA). 12-14 days after sensitization. Antigen challenge was performed by intratracheal instillation of OVA. Bronchial alveolar lavage of sensitized rats was performed immediately, 1h, 4h and 16h after OVA challenge. AMs were isolated by adherence. IL-1 activity was assayed by the comitogenic activity of the AM supernatants (only medium-cultured AM supernatants or lipopolysaccharide-stimulated AM supernatants) on ConA-stimulated murine thymocytes.Results:AMs obtained by BAL 1h and 4h after OVA challenge in sensitized rats simultaneously release significantly increased IL-1 activity ( each P<0.01). There was no significantly increased IL-1 bioactivity in only medium-cultured AM supernatants between any pair of the other groups or between any pair of the five groups in LPS-stimulated AM culture supernatants (all P>0.05). Conclusion:AMs from asthmatic rats released simultaneously increased IL-1 activity. LPS-stimulated AMs produced no further increase of IL-1 activity.