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| NF-κB靶向性哑铃形“圈套”寡核苷酸对海马神经元及神经元样细胞中COX-2表达的影响 | |
| 引用本文： | 孙艺平,赵永顺,陈冬冬,邹伟,宫瑾,于德钦,宫德正,王冬梅,徐红,赵杰.NF-κB靶向性哑铃形“圈套”寡核苷酸对海马神经元及神经元样细胞中COX-2表达的影响[J].中国药理学通报,2008,24(11). |
| 作者姓名： | 孙艺平 赵永顺 陈冬冬 邹伟 宫瑾 于德钦 宫德正 王冬梅 徐红 赵杰 |
| 作者单位： | 1. 大连医科大学生理学教研室,辽宁,大连,116044;大连医科大学机能学实验室,辽宁,大连,116044 2. 大连医科大学生理学教研室,辽宁,大连,116044 3. 辽宁师范大学生物系,辽宁,大连,116023 4. 大连医科大学机能学实验室,辽宁,大连,116044 |
| 基金项目： | 国家自然科学基金，辽宁省教育厅资助项目 |
| 摘 要： | 目的设计合成靶向性NF-κB的哑铃形圈套ODNs,并检测其对神经元样细胞及海马神经元中NF-κB及环氧化酶-2(COX-2)的抑制作用。方法采用NF-κB靶向性哑铃形圈套ODNs转染培养的PC12细胞,用Western blot方法对PC12细胞内NF-κB和COX-2的蛋白表达进行分析;转染培养的海马神经元再经LPS刺激后,用RT-PCR法检测COX-2mRNA。结果经靶向性NF-κB哑铃形圈套ODNs处理的PC12细胞中NF-κB的表达明显降低(P<0.01),同时伴有COX-2表达明显减少(P<0.01);海马神经元中COX-2mR-NA的表达明显降低(P<0.01)。结论靶向性NF-κB的哑铃形圈套ODNs可抑制神经元样细胞及神经细胞中NF-κB和COX-2的表达,且NF-κB对COX-2具有调控作用。 |
| 关 键 词： | NF-κB 哑铃形圈套ODNs 环氧化酶-2 海马神经元 PC12细胞 |
Inhibitory effect of circular dumb bell decoy ODNs targeting NF-κB on the expression of cyclooxygenase-2 in PC12 cells and hippocampal neurons |
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| SUN Yi-ping,ZHAO Yong-shun,CHEN Dong-dong,ZOU Wei,GONG Jin,YU De-qin,GONG De-zheng,WANG Dong-mei,XU Hong,ZHAO Jie.Inhibitory effect of circular dumb bell decoy ODNs targeting NF-κB on the expression of cyclooxygenase-2 in PC12 cells and hippocampal neurons[J].Chinese Pharmacological Bulletin,2008,24(11). | |
| Authors: | SUN Yi-ping ZHAO Yong-shun CHEN Dong-dong ZOU Wei GONG Jin YU De-qin GONG De-zheng WANG Dong-mei XU Hong ZHAO Jie |
| Institution: | SUN Yi-ping1,3,ZHAO Yong-shun1,CHEN Dong-dong2,ZOU Wei2,GONG Jin1,YU De-qin1,GONG De-zheng3,WANG Dong-mei3,XU Hong3,ZHAO Jie1(1.Dept of Physiology,3.Dept of Medical Functions,Dalian Medical University,Dalian Liaoning 116044,China,2.Dept of Biology,Liaoning Normal University,Dalian Liaoning 116023,China) |
| Abstract: | Aim To investigate the inhibitory effects of circular dumb bell decoy oligodeoxynucleotides(ODNs)targeting NF-κB on the expression of NF-κB and Cyclooxygenase-2(COX-2)in hippocampal neurons and PC12 cells.Methods PC12 cells and hippocampal neurons were transfected circular dumb bell decoy ODNs targeting NF-κB.The expression of NF-κB and COX-2 was observed in PC12 cells with Western blot analysis.The expression of COX-2 mRNA was detected in hippocampal neurons by RT-PCR analysis after they were stimulated by LPS.Results Circular dumb bell decoy ODNs could obviously inhibit the expression of NF-κB and COX-2 in the PC12 cells and COX-2 mRNA in the hippocampal neurons.Conclusion Circular dumb bell decoy ODNs targeting NF-κB could effectively inhibit the expression of NF-κB and COX-2 in the PC12 cells and neurons in which the NF-κB regulated the expression of COX-2. |
| Keywords: | nuclear factor kappa B circular dumb bell decoy oligodeoxynucleotides cyclooxygenase-2 hippocampal neurons PC12 cells |
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