Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine |
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Authors: | Chu Chun-Yen Wang Shiang-Yiu Chen Zeng-Weng Chien Maw-Sheng Huang Ji-Ping Chen Jen-Jin Hong Li-Shian Shiau Ai-Li Tsai Jeng-Liang Wu Chao-Liang |
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Affiliation: | Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 1 Shuehfu Road, Neipu, Pingtung 91201, Taiwan. cychu@mail.npust.edu.tw |
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Abstract: | In this study, we exploited a crp (cAMP receptor protein) gene-deleted, virulence plasmid-cured Salmonella choleraesuis mutant with decreased carbon source utilization, designated S.C.-Deltacrp/vpl(-), as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses of S.C.-Deltacrp/vpl(-) live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from Salmonella infection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developed Salmonella specific T-cell proliferative response in vitro. Our results indicate that immunogenic antigens in S.C.-Deltacrp/vpl(-) can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new live S. choleraesuis vaccine. |
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