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monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson''s disease
Authors:Etienne Hirsch   Merle Ruberg   Mireille Dardenne   Marie-Madeleine Portier   France Javoy-Agid   Jean-Francois Bach  Yves Agid  
Affiliation:1. I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France;2. I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France;3. Laboratoire de Biochimie Cellulaire, Collège de France, 75005 Paris France;1. Department of Pharmacology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, Fukushima, 960-1295, Japan;2. Emergency and Critical Care Medical Center, Fukushima Medical University Hospital, 1 Hikarigaoka, Fukushima, Fukushima, 960-1295, Japan;1. European Molecular Biology Laboratory, Structural and Computation Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany;2. Molecular Cancer Research Group, Institute of Medical Biology, University of Tromsø – The Arctic University of Norway, 9037 Tromsø, Norway;1. IRCCS Neuromed, Pozzilli (IS) 86077, Italy;2. Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy;3. Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via Roma 55, 56126 Pisa, Italy;4. Research Institute on Terrestrial Ecosystems (IRET), UOS Naples-CNR, Via Pietro Castellino 111, 80131 Naples, Italy;5. Department of Pediatrics Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA;6. Center for Host–Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York 10032, NY, USA;7. Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37240, USA;8. School of Health Sciences, Purdue University, West Lafayette, IN 47907-2051, USA;9. Department of Physiology and Pharmacology, Sapienza Rome University, Rome 00185, Italy;10. Division for Neurodegenerative Diseases, Department of Neurology, Mannheim Center for Translational Neuroscience, Medical Faculty Mannheim Heidelberg University, Mannheim 68167, Germany;1. Grupo VIENAP, Instituto de Ciencias de la Vid y del Vino (CSIC, Gobierno de La Rioja, Universidad de La Rioja). Carretera de Burgos, Km. 6. 26007 Logroño, Spain;2. Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC). Campus Universitario de Espinardo, Ed. 25. 30100 Murcia, Spain;1. Department of Neurology, University Medical Center, Georg-August University, Göttingen D-37075, Germany
Abstract:Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleus containing Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.
Keywords:Lewy body   Parkinson's disease   monoclonal antibody   substantia nigra   Purkinje cell
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