ＸＲＣＣ１、ＤＮＡ ＰＫｃｓ和ＰＣＮＡ在非小细胞肺癌中的表达及其与放疗疗效的关系研究

目的　探讨Ｘ线修复交叉互补基因（ＸＲＣＣ１）、ＤＮＡ ＰＫｃｓ和增殖细胞核抗原（ＰＣＮＡ）在非小细胞肺癌（ＮＳＣＬＣ）中的表达及其与放疗疗效的相关性，为预测ＮＳＣＬＣ放疗的敏感性提供理论依据。方法　６２例经病理活检证实的ＮＳＣＬＣ患者行三维适形放射治疗，放疗前后胸部增强ＣＴ扫描，按照ＷＨＯ实体瘤近期疗效标准进行评价；用免疫组化染色法检测放疗前ＮＳＣＬＣ组织中ＸＲＣＣ１、ＤＮＡ ＰＫｃｓ和ＰＣＮＡ蛋白的表达情况；将放疗的近期疗效与３种蛋白免疫组化结果进行统计分析。结果　按ＸＲＣＣ１蛋白（－）、（＋）、（＋＋）、（＋＋＋）分组经放疗后有效率（ＣＲ＋ＰＲ）分别为７０.０％、６６.７％、６４.０％、４０.０％，组间比较差异有统计学意义（P＜０.０５）；按ＤＮＡ-ＰＫｃｓ蛋白（－）、（＋）、（＋＋）、（＋＋＋）分组经放疗后有效率分别为８５.７％、７６.５％、５５.６％、２７.３％，组间比较差异有统计学意义（P＜０.０５）；按ＰＣＮＡ蛋白（－）、（＋）、（＋＋）、（＋＋＋）分组经放疗后有效率分别为０、３８.５％、６０％、７０.８％，组间比较差异有统计学意义（Ｐ＜０.０５）。结论　ＸＲＣＣ１、ＤＮＡ-ＰＫｃｓ蛋白表达与ＮＳＣＬＣ三维适形放射治疗的近期疗效呈负相关，ＰＣＮＡ蛋白表达与其呈正相关，检测这３种蛋白的表达在一定程度上可作为预测ＮＳＣＬＣ三维适形放射治疗的近期疗效指标。

Association between XRCC1,DNA-PKcs, and PCNA and radiotherapeutic effect in non-small cell lung cancer

Objective To investigate expressions of XRCC1,DNA-PKcs and PCNA proteins in non-small cell lung cancer（NSCLC）and its relationship to the effect of radiotherapy.Furthermore,to analyze the predict value of radiotherapy effect.Methods There were 62 patients with NSCLC who were treated by 3D-radiotherapy.Before and after the radiotherpy,they were checked by chest enhanced-CT to evaluate the short-term effect.The expression of XRCC1,DNA-PKcs and PCNA in 62 patients with NSCLC were determined by immunohistochemical method.The expression levels of the three kinds of proteins with the short-term radiotherapy effect were analyzed.Results The expressions of XRCC1,DNA-PKcs and PCNA were closely related with short-term effect after radiotherapy in NSCLC.The short-term effect was descent along with enhancement of XRCC1 and DNA-PKcs,and was rised along with enhancement of PCNA.Conclusion These findings indicated that the expressions of XRCC1,DNA-PKcs and PCNA may predict short-term effect of radiotherapy.The three proteins may have relationship with radiotherapeutic sensitivity in some extent.