| 甲型肝炎病毒单克隆抗体细胞株的建立及鉴定 |
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| 引用本文： | 黄建锋,邓德坚,张晓琼.甲型肝炎病毒单克隆抗体细胞株的建立及鉴定[J].海南医学,2011,22(21):1-3. |
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| 作者姓名： | 黄建锋 邓德坚 张晓琼 |
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| 作者单位： | 深圳市龙岗区沙湾医院,广东 深圳,518114 |
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| 摘 要： | 目的 探讨建立和鉴定甲型肝炎(HAV)病毒单克隆抗体(McAb)细胞株的方法,为临床提供抗体来源.方法 通过McAb杂交瘤细胞培养方法进行甲型肝炎病毒单克隆抗体细胞株的培养后,选取实验培养的具有稳定性的McAb杂交瘤细胞后编号,对各细胞株进行特异性、效价的测定,并进行比对.结果 McAb杂交瘤细胞与甲肝病毒抗原的结合特...
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| 关 键 词： | 甲型肝炎 病毒 单克隆抗体细胞株 临床鉴定 |
Establishment and identification of monoclonal antibody cell lines against hepatitis A virus |
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| HUANG Jian-feng,DENG De-jian,ZHANG Xiao-qiong.Establishment and identification of monoclonal antibody cell lines against hepatitis A virus[J].Hainan Medical Journal,2011,22(21):1-3. |
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| Authors: | HUANG Jian-feng DENG De-jian ZHANG Xiao-qiong |
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| Institution: | HUANG Jian-feng,DENG De-jian,ZHANG Xiao-qiong.Shawan Hospital in Longgang District of Shenzhen City,Shenzhen 518114,Guangdong,CHINA |
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| Abstract: | Objective To discuss the establishment and identification of monoclonal antibody(McAb) cell lines against hepatitis A virus(HAV).Methods The McAb cell lines against HAV were cultured.The stable McAb hybridoma cell lines were selected and numbered with A,B,C,which was then detected for specificity and titer.Results The titer of McAb hybridoma cell lines and HAV antigen was 1:(10 000~80 000).The titer of McAb cell culture supernatant and HAV antigen was 1:(2 048~1 6824).Conclusion The McAb hybridoma cell line... |
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| Keywords: | Hepatitis A Virus Monoclonal antibody cell lines Clinical identification |
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