| 弓形虫微线体蛋白8胞质尾蛋白的表达及纯化 |
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| 引用本文： | 尹志奎,张崇,郑斌.弓形虫微线体蛋白8胞质尾蛋白的表达及纯化[J].现代预防医学,2012,39(17):4491-4493. |
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| 作者姓名： | 尹志奎 张崇 郑斌 |
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| 作者单位： | 1. 新乡医学院药学院,河南新乡,453000
2. 新乡医学院寄生虫学教研室 |
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| 基金项目： | 河南省科技攻关计划项目(112102310209);新乡医学院博士科研启动基金(2010)资助 |
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| 摘 要： | 目的 制备弓形虫微线体蛋白8羧基端胞质尾(MIC8t)蛋白片段.方法 以弓形虫基因组为模板,PCR扩增MIC8t基因片段,构建MIC8t/pGEX-4T-1原核表达系统；IPTG诱导表达GST-MIC8t融合蛋白;亲和层析纯化融合蛋白.结果 构建了MIC8t原核表达系统,表达并纯化了GST-MIC8t融合蛋白.结论 在体外制备并纯化了GST-MIC8t融合蛋白,为后续研究MIC8t的功能奠定了基础.
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| 关 键 词： | 刚地弓形虫 微线体蛋白8胞质尾 蛋白表达 |
Expression on the C-terminal cytosolic tail domain of Toxoplasma gondii microneme protein 8 |
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| YIN Zhi-kui,ZHANG Chong,ZHENG Bin.Expression on the C-terminal cytosolic tail domain of Toxoplasma gondii microneme protein 8[J].Modern Preventive Medicine,2012,39(17):4491-4493. |
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| Authors: | YIN Zhi-kui ZHANG Chong ZHENG Bin |
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| Institution: | .School of Pharmacy,Xinxiang Medical University,Xinxiang,Henan 453000,China |
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| Abstract: | OBJECTIVE To produce the C-terminal cytosolic tail domain of microneme protein 8(MIC8t).METHODS MIC8t gene was obtained from genomic DNA by PCR amplification and sub cloned to vector pGEX-4T-1 to generate the translational fusion with GST.The recombinant protein GST-MIC8t was expressed in E.coli upon IPTG induction and then purified with affinity chromatography.RESULTS The recombinant plasmid pGEX-4T-1/MIC8t was constructed successfully and expressed as a fusion protein GST-MIC8t.CONCLUSION The purified fusion protein GST-MIC8t is obtained,which may provide the foundation for the further studies on the function of MIC8t. |
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| Keywords: | Toxoplasma gondii C-terminal cytosolic tail domain of microneme protein 8 Protein expression |
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