茶树精油对铜绿假单胞杆菌生物膜的抑制作用

目的 观察茶树精油对铜绿假单胞杆菌生物膜(BF)的抑制作用.方法 采用二倍稀释法测定茶树精油对铜绿假单胞杆菌的最小抑菌浓度(MIC),以一次性输液管和微孔滤膜作为生物膜吸附载体,用改良后的Brown平板连续培养法制备铜绿假单胞杆菌生物膜体外模型,采用光学高倍显微镜连续观察生物膜的形成,采用扫描电子显微镜进行生物膜结构观察和鉴定,以琼脂平板菌落计数法测定茶树精油对受试菌生物膜的抑制效果.结果 茶树精油对铜绿假单胞杆菌的MIC(孟乙烯4-ol浓度含量)为9.6μg/ml; 0.5倍MIC (4.8 μg/ml)、1倍MIC(9.6 μg/ml)虽能使成熟铜绿假单胞杆菌生物膜菌落数呈梯度性减少,但不能完全清除生物膜细菌,而2倍MIC(19.2 μg/ml)对细菌生物膜作用20 h后可完全清除生物膜细菌,3倍MIC(28.8μg/ml)则可在4h内完全清除生物膜.结论 茶树精油对铜绿假单胞菌生物膜有清除效应,可抑制生物膜形成,但需进一步研究验证.

Restraining effects of tea tree oil to the Pseudomonas aeruginosa biofilm

OBJECTIVE To find out and study the restraining effect of tea tree oil to Pseudomonas aeruginos biofilm.METHODS PVC biomedical material and millipore was used as adsorption carrier of biofilm and double-dilution method was used to detect the MIC of tea tree oil on Pseudomonas aeruginos,the in vitro model of Pseudomonas aeruginos biofilm was built by continuous development method on Brown’s plate,and scanning with electron microcopy(SEM)was used to evaluate the formation of biofilm.The viable bacterial colony was counted on the plate of agar so as to evaluate the restraining effect of tea tree oil for the tested bacterial biofilm.RESULTS The MIC of tea tree oil on Pseudomonas aeruginos(the concentration of Terpinen 4-ol)was 9.6 μg/ml.The concentration of tea tree oil of half of MIC(4.8 μg/ml),and one of MIC(9.6 μg/ml)can lower the number of the viable bacterial of the formed Pseudomonas aeruginos biofilm.For example,the concentration of tea tree oil of half of MIC can decrease the number of bacterial to 50×103,and one of MIC can decrease the number of bacterial to 12×103,bacterial biofilm could be cleared in 24 hours.However,twice of MIC(19.2 μg/ml)can break up the biofilm completely after 24 hours,and three times of MIC may clear bacterial biofilm during 4h.CONCLUSION Tea tree oil has a strong effect of clearing for Pseudomonas aeruginos biofilm step by step.However,some clinic pharmacy research will still needed to been done.