过敏性紫癜患儿急性期血清IL-10、IL-13、IL-15水平的变化及意义

目的分析过敏性紫癜(HSP)患儿急性期血清白细胞介素(IL)-10、IL-13、IL-15的水平变化及其与部分免疫学指标的关系,探讨其临床意义。方法取40例已确诊的HSP患儿血清,其中19例为有肾损害组,21例为无肾损害组;同时取20例健康儿童的血清。采用双抗体夹心ELISA法分别检测3组血清IL-10、IL-13、IL-15的含量。结果 HSP患儿血清IL-10、IL-13、IL-15水平均显著高于正常对照组(P<0.01),肾损害组血清IL-10、IL-13、IL-15水平均明显高于无肾损害组(P<0.05);HSP组IgA含量显著高于对照组,且肾损害组IgA含量明显高于无肾损害组(P<0.05)。经相关性分析IL-10、IL-13、IL-15与IgA明显相关。结论 HSP存在细胞免疫与体液免疫功能紊乱,血清IL-10、IL-13、IL-15及IgA与HSP、紫癜性肾炎(HSPN)的发病过程密切相关。

Changes of Serum IL-10,IL-13,IL-15 in Children with Henoch-schonlein Purpura During Acute Phase

Objective To analyze the changes of serum interleukin IL-10,IL-13,IL-15 in children with henoch-schonlein purpura during acute phase and the relationship with partial immunological indexes.Methods 40 serum samples in cases with HSP were taken,including 19 cases with renal involvement and 21 cases without renal involvement.20 serum samples in healthy children were taken.Serum levels of IL-10,IL-13,IL-15 were detected respectively by ELISA.Results IL-10,IL-13,IL-15 levels in children with HSP during acute phase were significantly higher than that of normal control group(P < 0.01);IL-10,IL-13,IL-15 levels with renal involvement were significantly higher than that of without renal involvement(P < 0.05);IgA levels in children with HSP were significantly higher than that of control group,and IgA levels with renal involvement were significantly higher than that of without renal involvement(P < 0.05);IL-10,IL 13,IL-15 and IgA showed signifincant correlation.Conclusion IL-10,IL-13,IL-15 and IgA have related closely to the pathogenesis of HSP and HSPN.There is dysfunction of Cellular immune and Humoral immune in children with henoch-schonlein purpura.