COX-2和MMP-9在绝经前后子宫内膜息肉中的表达及其意义

目的研究绝经前、后子宫内膜息肉(EMP)中环氧合酶-2(COX-2)、基质金属蛋白酶-9(MMP-9)的表达及二者的相关性,为研究EMP形成机理提供线索。方法选取存档石蜡标本共150例,其中绝经前、后EMP各45例,绝经前正常增生期子宫内膜和绝经后正常萎缩型子宫内膜各30例,用免疫组化SP法检测各组COX-2、MMP-9的表达情况,并分析两指标在EMP组织中的表达及相关性。结果绝经前组COX-2、MMP-9在EMP腺体和间质的表达均显著高于二者在正常增生期子宫内膜中的表达(P<0.05)。绝经后组COX-2、MMP-9在EMP间质和腺体中的表达均高于二者在绝经后正常萎缩型子宫内膜中的表达(P<0.05)。COX-2在绝经前、后EMP腺体中的表达差异无统计学意义(P>0.05),COX-2在绝经前EMP间质中的表达高于其在绝经后EMP间质中的表达(P<0.05),MMP-9在绝经前EMP的腺体和间质中的表达高于其在绝经后的表达(P<0.05)。COX-2与MMP-9在EMP腺体和间质表达呈正相关(P<0.01)。结论 COX-2和MMP-9在EMP中高表达,且呈正相关。

Expression of COX-2 and MMP-9 in Patients with EMP in Pre-and Post-menopausal Women

Objective To investigate the expression of Cyctooxygenase-2(COX-2),matrix metalloproteinase-9(MMP-9)in patients with endometrial polyps(EMP) in pre-and post-menopausal women and their relationship,and to investigate the pathogenesis of EMP.Methods 150 cases with EMP(45 from pre-menopausal women and the other 45 from post-menopausal women) and 60 cases with normal endometrium(30 were proliferative phase endometrium and the other 30 were atrophic endometrium) were collected.Immunohistochemical SP method was used to detect the expression of COX-2 and MMP-9 in each tissue.Results 1.In Premenopausal group: Expression of COX-2 and MMP-9 in epithelial cells and stroma of EMP were all significantly higher than those in the normal proliferative phase endometrium(P<0.05).2.In Postmenopausal group: Expression of COX-2 and MMP-9 in epithelial cells and stroma of EMP were all significantly higher than those in the normal atrophic endometrium(P<0.05).3.Stromal expression of COX-2 and MMP-9 were found higher in premenopausal polyps compared to postmenopausal polyps(P<0.05);Epithelium expression of MMP-9 were also found higher in premenopausal polyps compared to postmenopausal polyps(P< 0.05).There were no other significant differences in the immunohistochemical expression of COX-2 in the epithelium of premenopausal and postmenopausal endometrial polyps(P>0.05).4.There was a significantly positive correlation between expression of COX-2 and MMP-9(r=0.6135,P<0.01).Conclusion The expression of COX-2 is closely associated with MMP-9.Overexpression of COX-2 and MMP-9 in the EMP suggested that COX-2 and MMP-9 may play a role in development of EMP.The pathogenesis of pre-and postmenopausal endometrial polyps may be difference,which may be related to estrogen levels.