Protective effect of green tea extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney |
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| Authors: | Sara A Khan Shubha Priyamvada Neelam Farooq Sheeba Khan Md Wasim Khan Ahad NK Yusufi |
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| Institution: | 1. Department of Biophysics and Biochemistry, Dnipropetrovs''k National University, Gagarin Ave. 72, Dnipropetrovs''k 49050, Ukraine;2. Department of Inorganic Chemistry, Taras Shevchenko University, Kiev 01033, Ukraine;3. Department of Inorganic Chemistry, State Higher Education Establishment “Ukrainian State University of Chemical Technology”, Gagarin Ave. 8, Dnipropetrovs''k 49005, Ukraine;4. Department of Chemistry, Texas A&M University, PO Box 30012, College Station, TX 77842-3012, USA;1. Department of Physiology, University of Ni?, Faculty of Medicine, Bulevar Dr Zoran Djindji? 81, Ni?, Serbia;2. Department of Pharmacy, University of Ni?, Faculty of Medicine, Bulevar Dr Zoran Djindji? 81, Ni?, Serbia;3. Department of Histology, University of Ni?, Faculty of Medicine, Bulevar Dr Zoran Djindji? 81, Ni?, Serbia;4. Department of Anatomy, University of Ni?, Faculty of Medicine, Bulevar Dr Zoran Djindji? 81, Ni?, Serbia;1. Nutritional Sciences, 301 Human Sciences, Oklahoma State University, Stillwater, OK 74078-6141, USA;2. Harold Hamm Diabetes Center and Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center (OUHSC), OKC, OK 73104, USA;1. Medicinal Chemistry and Pharmacology Division, Indian Institute of Chemical Technology (IICT), Hyderabad 500 007, India;2. National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad 500 037, India;3. National Centre for Mass Spectrometry, Indian Institute of Chemical Technology (IICT), Hyderabad 500 007, India;4. CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad 500 007, India;5. Faculty of Pharmacy, Osmania University, Hyderabad 500 007, India |
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| Abstract: | Gentamicin (GM) is an effective aminoglycoside antibiotic against severe infections but nephrotoxicity and oxidative damage limits its long term clinical use. Various strategies were attempted to ameliorate GM nephropathy but were not found suitable for clinical practice. Green tea (GT) polyphenols have shown strong chemopreventive and chemotherapeutic effects against various pathologies. We hypothesized that GT prevents GM nephrotoxicity by virtue of its antioxidative properties. A nephrotoxic dose of GM was co-administered to control and GT-fed male Wistar rats. Serum parameters and enzymes of oxidative stress, brush border membrane (BBM), and carbohydrate metabolism were analyzed. GM increased serum creatinine, cholesterol, blood urea nitrogen (BUN), lipid peroxidation (LPO) and suppressed superoxide dismutase (SOD) and catalase activities in renal tissues. Activity of hexokinase, lactate dehydrogenase increased whereas malate dehydrogenase decreased. Gluconeogenic enzymes and glucose-6-phosphate dehydrogenase were differentially altered in the cortex and medulla. However, GT given to GM rats reduced nephrotoxicity parameters, enhanced antioxidant defense and energy metabolism. The activity of BBM enzymes and transport of Pi declined by GM whereas GT enhanced BBM enzymes and Pi transport. In conclusion, green tea ameliorates GM elicited nephrotoxicity and oxidative damage by improving antioxidant defense, tissue integrity and energy metabolism. |
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