| 内质网应激对树突状细胞表面晚期糖基化终产物受体表达的影响 |
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| 引用本文： | 祝筱梅,姚咏明,董宁,张庆红,于燕,盛志勇. 内质网应激对树突状细胞表面晚期糖基化终产物受体表达的影响[J]. 感染、炎症、修复, 2011, 12(4): 197-201 |
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| 作者姓名： | 祝筱梅 姚咏明 董宁 张庆红 于燕 盛志勇 |
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| 作者单位： | 解放军总医院第一附属医院烧伤研究所,北京,100048 |
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| 基金项目： | 国家自然科学基金项目（81071545,30800437,81130035,81121004）;国家重点基础研究发展计划项目（2012CB518102）;创伤、烧伤与复合伤国家重点实验室开放课题（SKLKF201103,SKLKF201002） |
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| 摘 要： | 目的:晚期糖基化终产物受体(RAGE)与其配体结合在炎症及免疫反应中具有重要作用.本研究探讨内质网应激(ERS)在高迁移率族蛋白B1(HMGB1)诱导树突状细胞(DC)表面RAGE上调中的作用及意义.方法:分离正常BALB/c小鼠脾脏DC进行体外培养,给予HMGB1刺激后检测DC表面RAGE表达水平及细胞ERS相关分子...
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| 关 键 词： | 脓毒症 树突状细胞 晚期糖基化终产物受体 内质网应激 |
Effects of endoplasmic reticulum stress on up-regulation of the receptor for advanced glycation endproducts on surface of dendritic cells |
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| Affiliation: | Zhu Xiaomei,Yao Yongming,Dong Ninget al. Department of Microbiology and Immunology, Burns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, China |
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| Abstract: | Objective： To clarify the involvement of endoplasmic reticulum stress （ERS） in up-regulation of receptor for advanced glycation endproduets （RAGE） on surface of dendritic cells （IX;） induced by high mobility group box-1 protein （HMGB1）. Methods：DCs were isolated from the spleens of male BALB/c mice. Expressions of ERS related molecules were analyzed by Western blot analysis, and the expression of RAGE on surface of DC was determined by flow cytometry after HMGB1 stimulation. The effect of tunicamycin （0. 1,1,10 μg/ml for 24, 48,72 hours） on RAGE expression was also assessed by flow cytometry. Results： HMGB1 induced up-regulation of RAGE expression and significant ERS response in murine splenic DCs. Pharmacological ERS （induced by tunicamycin） resulted in enhanced expression of RAGE on surface of DCs. Conclusions： These results indicate that ERS is crucial for up-regulation of RAGE on surface of DCs. |
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| Keywords: | Sepsis Dendritic ceils Receptor for advanced glycation endproducts Endopiasmie reticulum stress |
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