甲硝唑滴眼液与角膜接触镜多功能护理液体外对棘阿米巴原虫的杀伤效果

目的 了解6种角膜接触镜多功能护理液和加入甲硝唑滴眼液的护理液对自生生活性棘阿米巴原虫的杀伤效果。方法 将6种多功能护理液分别加入96孔培养板中,每种护理液占用48孔,其中24孔滴入棘阿米巴悬液,另外24孔先滴入甲硝唑滴眼液后再滴入棘阿米巴悬液,室温静置8 h后在倒置显微镜下观察棘阿米巴的形态变化和数量。将残存的棘阿米巴原虫分别在PYG培养液中培养5 d,观察其形态、活性与增殖能力的变化。结果 单纯护理液组1～6号棘阿米巴原虫检出率分别为0、8.3%、29.1%、41.7%、62.5%、79.2%,加入甲硝唑滴眼液后护理液1～6号棘阿米巴原虫检出率分别为0、0、4.2%、8.3%、16.7%、16.7%,3～6号护理液加与不加甲硝唑滴眼液杀伤棘阿米巴原虫的效果差异有统计学意义（χ^2=3.75～18.78,P〈0.05）。残存的棘阿米巴原虫经培养后活力与增殖力减弱。结论 部分多功能护理液对棘阿米巴原虫的杀伤效果不佳,添加甲硝唑滴眼液后杀伤效果明显提高。

THE KILLING EFFICIENCY OF ARILIN AND CONTACT LENS SOLUTIONS ON ACANTHAMOEBA

Objective To analyze the killing efficiency of six kinds of contact lens solutions and solutions with arilin on free-living Acanthamoeba. Methods The solutions were added into 96-well microtiter plates respectively. Each care solution used 48 holes,24 of those holes were added with suspension of Acanthamoeba. The other 24 holes were added in arilin and suspension of Acanthamoeba. After standing in room temperature for eight hours, the morphologic changes and quantity of the remn, ant Acanthamoeba were observed under an inverted microscope. The remnant Acanthamoeba were cultivanted in PYG-culture medium for five days. Then, their variation of appearance, activity and reproductive activity were observed. Results In the six experimental groups using contact lens solutions only, the detection rates of Acanthamoeba of were 0,8.3%, 29.1% ,41.7% ,62.5% and 79.2 %, respectively. After arilin was added, the detection rates of Acanthamoeba of those six groups were 0,0,4.2 %,8.3%, 16. 7% and 16.7%, respectively. From group 3 to group 6, after added in arilin, the differences of the killing efficiency of contact lens solutions were signficant （Х^2= 3.75-18.78 ,P〈0. 05）. Cultivation of the remnant Acanthamoeba showed a reduction in their activity and proliferative ability. Conclusion The killing efficiency of some contact lens solutions on free-living Acanthamoeba is not satisfied. Arilin can improve the killing efficiency of contact lens solutions.