| 巢式PCR检测胃癌患者外周血CK19 mRNA的临床意义 |
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| 引用本文： | 王永强,欧阳晓辉,王万祥.巢式PCR检测胃癌患者外周血CK19 mRNA的临床意义[J].内蒙古医学杂志,2011,43(3):268-270. |
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| 作者姓名： | 王永强 欧阳晓辉 王万祥 |
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| 作者单位： | 1. 内蒙古自治区人民医院普外二科,内蒙古,呼和浩特,010017
2. 内蒙古医学院附属医院肝胆外科,内蒙古,呼和浩特,010059 |
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| 摘 要： | 目的：对外周血CK19 mRNA基因表达的检测,探讨对胃癌患者微转移的诊断价值。方法：采用巢式RT-PCR方法检测63例胃癌患者,20例健康志愿者外周血CK19 mRNA的表达情况。结果：胃癌患者外周血中CK19 mRNA的阳性检出率为36.5%,并与肿瘤TNM分期、肿瘤浸润深度有关（P〈0.05）,而与年龄、性别、肿瘤分化程度、肿瘤大小、有无淋巴结转移无关（P〉0.05）,而健康志愿者外周血中CK19 mR-NA无1例阳性表达。结论：CK19 mRNA可以作为判断胃癌患者外周血微转移的肿瘤标志物。
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| 关 键 词： | 胃癌 微转移 CK19 mRNA 细胞角蛋白 RT-PCR |
The Clinical Significance of Nested PCR Detection of CK19 mRNA in Peripheral Blood of Gastric Cancer Patients |
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| WANG Yong-qiang,OUYANG Xiao-hui,WANG Wan-xiang.The Clinical Significance of Nested PCR Detection of CK19 mRNA in Peripheral Blood of Gastric Cancer Patients[J].Inner Mongolia Medical Journal,2011,43(3):268-270. |
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| Authors: | WANG Yong-qiang OUYANG Xiao-hui WANG Wan-xiang |
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| Institution: | 1.Department of General Surgery,Inner Mongolia Autonomaus Region People＇s Hospital,Huhhot 010017 China;2.Department of Hepatobiliary Surgery,The Affiliated Hospital of Inner Monglia Medical College,Huhhot 010050 China) |
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| Abstract: | Objective：CK19 mRNA in peripheral blood gene expression detection of micrometastases.In order to explore the micro-metastasis of gastric cancer diagnosis.Methods：The expression of CK19 mRNA in peripheral blood is detected by a nested RT-PCR of 63 cases of gastric cancer patients and 20 healthy volunteers.Results：CK19 mRNA in peripheral blood of patients with gastric cancer detection rate was 36.5% positive,and with tumor TNM stage,tumor depth of invasion（P0.05）,but not with age,gender,tumor differentiation,tumor size,lymph node Metastasis（P 0.05）,and CK19 mRNA in peripheral blood of healthy volunteers is none positive.Conclusion：CK19 mRNA as judged micrometastasis in patients with gastric cancer tumor markers. |
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| Keywords: | CK19 mRNA RT-PCR |
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