三磷酸腺苷-氯化镁对兔肺再灌注损伤的保护作用

目的利用家兔左肺灌注模型，探讨三磷酸腺苷一氯化镁ATP-MgCl2）对缺氧再灌注肺线粒体内钙离子浓度和肺组织水分的影响。方法观察ATP-MgCl2对再灌注肺组织线粒体内钙离子浓度和肺组织水分的影响。结果（1）家兔左肺灌注模型均获成功。（2）缺氧再灌注组线粒体内钙离子浓度显著高于正常对照和缺氧组（P〈0．01）；ATP-MgCl2加用再灌注组线粒体内钙离子浓度显著低于缺氧再灌注组（P〈0．01）。（3）缺氧再灌注组肺组织湿干重比值（LW／DR）显著高于正常对照和缺氧组（P〈0．01）；ATP-MgCl2加用再灌注组LW／DR显著低于缺氧再灌注组（P〈0．01）。结论本模型具有操作简便、成功率高的优点，是可行的肺离体研究模型；ATP-MgCl2明显抑制家兔缺氧再灌注肺线粒体内钙沉积和肺组织水肿，提示ATP-MgCl2对肺缺血再灌注损伤具有保护作用。

Protective Effects of ATP- MgCl2 on Reperfusion Injury in Rabbit Lung Perfusion Model

OBJECTIVE The purpose of this study is to establish reliable and appropriate rabbit left lung perfusion model and to clarify whether ATP-MgCl_(2) can influence calcium concentration in mitochondria during lung hypoxia/reperfusion injury in the model.METHODS First,we established rabbit single left lung perfusion model.Second,we use this model to clarify influence of ATP-MgCl_(2) on calcium concentration in mitochondria during lung reperfusion injury.RESULTS(1) Rabbit left lung perfusion model were successfully established in all 30 cases.(2) The free calcium concentration in mitochondria in hypoxia/reperfusion group was significantly higher than that in normal control and hypoxia groups(P<0.01);In ATP-MgCl_(2) added reperfusion group was significantly lower than that in hypoxia/reperfusion group(P<0.01);Lung wet/dry weight ratio in ATP-MgCl_(2) added reperfusion group was less than that in hypoxia and hypoxia/reperfusion groups(P<0.01).CONCLUSION The rabbit left lung perfusion model is a useful and reliable model for lung research.ATP-MgCl_(2) may have protective effect on the lung hypoxia/reperfusion injury through inhibition of mitochondrial calcium accumulation and reduction of lung tissue edema.