双琼脂MTT法测定肿瘤的药物敏感性

目的：采用将集落法和ＭＴＴ法结合发展而成的双琼脂ＭＴＴ法以克服ＭＴＴ法中存在下沉细胞干扰测定的缺点。方法；对人肿瘤细胞及正常细胞株在双琼脂ＭＴＴ法和ＭＴＴ法光密度，抗癌药抑制率进行比较及临床实体瘤用两种方法的抗癌药抑制率进行比较。结果：正常细胞在双琼脂ＭＴＴ法中生长不良，用该两种方法测定临床２０例食道癌对抗癌药抑制率，比较显示双琼脂ＭＴＴ法的抑制率大于ＭＴＴ法。结论：双琼脂ＭＴＴ法提高了药敏结果的

Analysis of chemosensitivity of tumor using double-layer agarose MTT method

AIM:To overcome the weakness of interfering by the normal cells in 3 (4,5 dimethylthiazol 2 yl) 2, 5 diphenyltetrazolium bromide (MTT) method, We used double layer agarose MTT (DMTT) method which was developed by combining colony method and MTT method. METHODS: We compared the inhibition rates of the antitumor drugs and the optical density between the human tumor cell lines and the normal cell lines by DMTT method and MTT method, and we also compared the inhibition rates of the antitumor drugs in clinical tumor between the 2 methods. RESULTS: The normal cells grew poorly in DMTT method. It demonstrated that the inhibition rates of the antitumor drugs determined by DMTT method were higher than that of determined by MTT method in 20 esophagus tumors. CONCLUSION: DMTT method improves the accuracy of chemosensitivity and is better than MTT method.