联合应用可乐定、美多心安、二氢麦角碱对大鼠重型颅脑损伤后血脑屏障通透性的影响

目的研究可乐定、美多心安、二氢麦角碱三药合用对血脑屏障通透性的影响以评估其疗效。方法应用Feeney法建立大鼠重型颅脑损伤模型,随后将重型颅脑损伤的大鼠随机分成三组:生理盐水组,甘露醇组,可乐定、美多心安、二氢麦角碱联合用药组。在伤后4h、6h、12h、24h、72h及7d,用化学定量法观测3组之间血脑屏障通透率的变化,并采用镧示踪法观察三组大鼠颅脑损伤后血脑屏障超微结构的变化。结果血脑屏障通透率在伤后7d三组之间无明显差异(P>0.05)。在其余时段中,生理盐水组和甘露醇组之间无明显差异(P>0.05),但三药联用组血脑屏障的通透性则明显低于前两组(P<0.05)。大鼠颅脑损伤后血脑屏障超微结构的变化与伤区脑组织血脑屏障通透率变化一致。结论对于重型颅脑损伤早期联合应用可乐定、美多心安、二氢麦角碱能降低血脑屏障通透率,减轻脑水肿。

Effect of Allied Application of Clonidine, Metoprolol and Ergoloid on Permeability of Blood Brain Barrier in Rats with severe Craniocerebral Injury

Objective To study the effects of allied application of clonidine, metoprolol and ergoloid on blood brain brarrier (BBB) permeability after the sever craniocerebral injury. Methods Ninety-nine Wistar rats with severe craniocerebral injury induced by Freeney method were randomly divided into 3 groups, i.e. normal saline group, mannitol group and allied application of clonidine, metoprolol and ergoloid group of 33 rats each. The BBB permeability was determined by chemical quantitative analysis in 5 rats of each group respectively 4, 6, 12, 24, 72 and 168 hours after the craniocerebral injury. The ultrastructure of BBB was observed by electron microscope in 1 rat of each group 4, 6 and 12 hours respectively after the craniocerebral injury. Results The permeating rate of BBB was significantly lower in the allied application group than that in the normal saline group and mannitol group 4, 6, 12, 24 and 72 hours after the craniocerebral injury (P<0.05). There was insignificant difference in the permeating rate of BBB among normal saline, mannital and allied application groups 168 hours after the injury (P>0.05). There was insignificant difference in the permeating rate of BBB between normal saline group and mannitol group 4, 6, 12, 24, 72 and 168 hours after the injury. The change in the ultrastructure of BBB after the injury was similar to that in the permeating rate of BBB. Conclusion The permeating rate of BBB can be decreaed and the brain edema can be relieved by early allied application of clonidine, metoprolol and ergoloid in the rats with severe craniocerebral injury.