Effects of nafamostat mesilate, somatostatin, and glucagon on caerulein-induced acute pancreatitis in rats |
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Authors: | Kikuo Mori Yoshihisa Marugami Yoichi Sakurai Masahiro Ochiai Shigeru Hasegawa Hiroki Imazu Toshiki Matsubara Masashi Suganuma Atsushi Shikata and Takahiko Funabiki |
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Institution: | (1) Department of Surgery, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, 470-11 Toyoake, Aichi, Japan |
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Abstract: | The inhibitory effects of somatostatin (SMS) and glucagon (Gn) on acute pancreatitis were evaluated in an experimental acute
pancreatitis model in male Wistar rats. The effects of these agents were compared with those of nafamostat mesilate (NM).
The acute pancreatitis was induced by four serial subcutaneous injections of caerulein. The rats were divided into four groups.
The first group (n=28) received SMS daily, the second group (n=28) received Gn daily, and the third group (n=28) received NM daily after the first injection of caerulein. The fourth group (n=42) received caerulein alone and served as the control group. Animals were sacrificed 4, 6, 8, 12, and 24 h, and 3 and 7
days after the first administration of caerulein and the degree of severity of the acute pancreatitis was evaluated by serial
morphological and histological examinations of pancreatic tissues, as well as in terms of the serum concentrations of amylase
and lipase. The characteristic findings of acute pancreatitis in the animals of all groups treated with SMS, Gn, or NM were
markedly attenuated at all time points after the treatments compared with findings in the controls (caerulein alone) in terms
of wet weight of pancreas, serum concentrations of amylase and lipase, formation of intracellular vacuoles in acinar cells,
interstitial edema, and infiltration of an inflammatory cell component. The inhibitory effects of SMS, Gn, and NM on acute
pancreatitis were similar at the doses used. These results suggest that SMS and Gn are as useful as NM, they may be of value
for the treatment of acute pancreatitis. |
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Keywords: | caerulein-induced pancreatitis experimental pancreatitis somatostatin |
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