右美托咪定对缺血-再灌注大鼠肠黏膜屏障功能的影晌

目的观察右美托咪定对肠缺血-再灌注损伤（IRI）大鼠肠型脂肪酸结合蛋白（I-FABP）及炎症因子的影响，并探讨其作用机制。方法建立大鼠肠IRI模型，随机分为假手术组、模型组、右美托咪定组。分别于缺血一再灌注后2h和6h时间点抽取大鼠腹主动脉血，采用酶联免疫吸附试验（ELISA）法检测大鼠血清I-FABP水平；于实验终点留取肠组织，ELISA法检测白介素-6（IL-6）和IL-10的含量，苏木精-伊红（HE）染色后经Chiu六级评分法判定大鼠小肠组织损伤程度。结果与假手术组比较，模型组和右美托咪定组再灌注2h和6h时血清I-FABP水平明显升高，差异有统计学意义（P〈0．05）；与模型组比较，右美托咪定组血清I-FABP含量显著下降，差异有统计学意义（P〈0．05）。模型组和右美托咪定组肠组织中IL-6和IL-10的含量均明显高于假手术组，差异有统计学意义（P〈0．05）；与模型组比较，右美托咪定组肠组织中IL-6含量降低，IL．10含量升高，差异均有统计学意义（P〈0．05）。右美托咪定组Chiu评分显著低于模型组，但高于假手术组，差异均有统计学意义（P〈0．05）。结论右美托咪定可有效减少肠缺血-再灌注后炎症因子的产生，降低血浆中I-FABP水平，具有肠道保护作用。

Effects of dexmedetomidine on function of intestinal mucosal barrier of rats with ischemia-reperfusion injury

Objective To observe the effects and to discuss the mechanism of dexmedetomidine on the intestinal fatty acid binding protein （I-FABP） and inflammatory factors of rats with intestinal ischemia-reperfusion injury. Methods The intestinal ischemia reperfusion injury model of rats was established and then rats were randomly divided into the sham operation group, ischemia-reperfusion group, and dexmedetomidine group. The aortic blood of rats was extracted at the 2 h and 6 h after ischemia-reperfusion. The plasma I-FABP levels were measured by ELISA. By the end of experiments, the contents of IL-6 and IL-10 in intestinal tissues were measured by ELISA. The intestinal tissues were stained by the HE and their injury severity was scored by the methods of Chin＇s six levels. Results The plasma I-FABP levels of the ischemia-reperfusion group and dexmedetomidine group at 2 h and 6 h after reperfusion were significantly higher than those of the sham operation group. The differences were statistically significant （P〈0.05）. Compared to the ischemia-reperfusion group, the plasma I-FABP levels of the dexmedetomidine group were significantly decreased. The differences were statistically significant （P〈0.05）. The contents of IL-6 and IL-10 in intestinal tissues of the ischemia- reperfusion group and dexmedetomidine group were significantly higher than the sham operation group. The differences were statistically significant （P〈0.05）. Compared to the ischemia-reperfusion group, the contents of IL-6 in intestinal tissues of the dexmedetomidine group were lower, while the contents of IL-10 were higher. The differences were statisdcaUy significant （19 〈 0. 05）. The Chiu＇s score of the dexmedetomidine group was significandy lower than that of the ischemia-reperfusion group but higher than that of the sham operation group.The differences were statistically significant （P 〈0.05）. the production of inflammatory factor after intestinal I-FABP, and protect the intestinal functions. Conclusion Dexmedetomidine can effectively reduce ischemia reperfusion, decrease the content of plasma