| 反相高效液相色谱法测定人胆汁中八种结合胆酸 |
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| 引用本文： | 戴智勇,谭桂山,钱康年,陈萱.反相高效液相色谱法测定人胆汁中八种结合胆酸[J].中南大学学报(医学版),1997(4). |
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| 作者姓名： | 戴智勇 谭桂山 钱康年 陈萱 |
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| 作者单位： | Department of Pharmacy，Xiangya Hospital，Hunan Medical University，Changsha 410008 |
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| 摘 要： | 采用ＨＰＬＣ法测定人胆汁中八种结合胆酸，色谱柱为ＹＷＧ－Ｃ１８（３μｍ），柱温３０℃，流动相为甲醇／水（６５／３３５，Ｖ／Ｖ，ｐＨ３．０），检测波长２１０ｎｍ。各结合胆酸在５０～１０００μｇｍｌ－１浓度范围呈良好的线性关系，回收率在９１．２％～１０８．６％之间。３０例胆囊结石和２０例无结石病人胆汁样本测定结果显示，二者在胆汁酸的构成上存在差异。
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| 关 键 词： | 色谱法.高效液相 结合胆酸 胆汁 |
SIMULTANEOUS DETERMINATION OF EIGHT KINDS OF CONJUNCT BILE ACIDS IN HUMAN BILE BY R HPLC |
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| Dai Zhiyong,Tan Guishan,Qian Kangnian,Chen Xuan.SIMULTANEOUS DETERMINATION OF EIGHT KINDS OF CONJUNCT BILE ACIDS IN HUMAN BILE BY R HPLC[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1997(4). |
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| Authors: | Dai Zhiyong Tan Guishan Qian Kangnian Chen Xuan |
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| Abstract: | |
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| Keywords: | chromatography high pressuer liquid conjucated bile acids bile |
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