| 家蝇对菊酯类杀虫剂抗性的分子生物学监测 |
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| 引用本文： | 史智勇,李富军,王绪明,杨银书,赵红斌.家蝇对菊酯类杀虫剂抗性的分子生物学监测[J].中华卫生杀虫药械,2008,14(4):260-261. |
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| 作者姓名： | 史智勇 李富军 王绪明 杨银书 赵红斌 |
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| 作者单位： | 1. 兰州军区总医院,甘肃,兰州,730050
2. 兰州军区疾病预防控制中心,甘肃,兰州,730020 |
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| 摘 要： | 目的用分子生物学方法测定甘肃省部分地区家蝇对菊酯类杀虫剂的抗药性。方法用引物P1、P2、P3对采自甘肃省6个地区（部队营区）的家蝇的钠离子通道基因进行扩增。结果4个地区的家蝇对菊酯类杀虫剂产生了抗性，其他2个地区的家蝇对菊酯类杀虫剂仍然敏感。结论PCR可用于家蝇抗药性的快速检测。
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| 关 键 词： | 家蝇 抗药性 PCR |
Molecular biological monitor of phyrethoid-resistance of house flies |
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| SHI Zhi-yong,LI Fu-jun,WANG Xu-ming,YANG Yin-shu,ZHAO Hong-bin.Molecular biological monitor of phyrethoid-resistance of house flies[J].Chinese Journal of Hygienic Insecticides and Equipments,2008,14(4):260-261. |
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| Authors: | SHI Zhi-yong LI Fu-jun WANG Xu-ming YANG Yin-shu ZHAO Hong-bin |
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| Affiliation: | SHI Zhi-yong1,LI Fu-jun1,WANG Xu-ming2,YANG Yin-shu2,ZHAO Hong-bin1 |
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| Abstract: | Objective To monitor the phyrethoid-resistance of house flies from 6 areas of Gansu province by molecular biological method.Methods Sodium channel genes of house flies from 6 areas of Gansu province(military districts) were amplified with 3 primers(P1,P2,P3).Results The house flies from 4 areas of Gansu province had developed resistance to phyrethoid,the house flies from other two areas of Gansu province were still sensitive to phyrethoid.Conclusion PCR method can be applied to detect the phyrethoid-resistance of house flies quickly. |
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| Keywords: | house fly resistance PCR |
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