骨髓单个核细胞缺血组织局部移植对血运重建的影响

目的：研究骨髓单个核细胞局部移植对缺血组织血运重建的影响。方法：从大鼠胫骨及股骨采集骨髓细胞，密度梯度离心法分离出骨髓单个核细胞。分离、切断近交系Wistar大鼠右侧股动脉及其分支，建立单侧后肢缺血模型。分7点在缺血后肢局部注射单个核细胞悬液（MNC组）或磷酸盐缓冲液（PBS组）。术后第2，4周测定各组后肢动静脉氧差（AVDO2）并取内收肌制作组织切片，免疫组织化学法分析血管新生。结果：术后第2，4周MNC组内收肌毛细血管密度、毛细血管与肌纤维比值高于PBS组（P<0.01），AVDO2低于同期PBS组（P<0.05）。结论：在大鼠缺血后肢局部直接植入骨髓单个核细胞可诱导新生血管形成，改善缺血组织的血流灌注。

Augmentation of revascularization by implantation of autologous bone marrow mononuclear cells in a rat ischemic hind limb model

OBJECTIVE: To examine whether the in vivo implantation of autologous bone-marrow mononuclear cells (BM-MNCs) can augment postnatal neovascularization in rat model of hind limb ischemia. METHODS: Rat BM-MNCs were isolated by centrifugation through a Ficoll-paque density gradient. The rat ischemic hind limb model was made by ligation of the right femoral artery and its branches of imbred Wistar rats. BM-MNCs (MNC group) or phosphate saline buffer (PBS group) were injected into 7 points of the ischemic muscles (20 microl/point). To assess angiogenesis, histologic evaluation was performed on tissue slices from adductor muscles with immunohistochemical staining at the 2nd and 4th weeks after the ligation. Severity of ischemic insult was evaluated by the femoral arteriovenous oxgen difference (AVDO2) at the 2nd and 4th weeks after the ligation. RESULTS: The MNC groups had a higher capillary density and higher capillary/muscle fiber ratios than that of the sham and PBS groups (P < 0.01) at the 2nd and 4th weeks after the ligation. AVDO2 of the MNC group significantly decreased compared with that of the PBS groups (P < 0.05). CONCLUSION: The implantation of autologous BM-MNCs induced neovascularization in a rat ischemic hind limb model. The implantation of autologous BM-MNCs augmented the collateral perfusion of ischemic hind limb.