莲房原花青素对乙醇诱导肝细胞损伤的拮抗作用

目的:研究莲房原花青素(LSPC)对乙醇诱导的人胚肝细胞株L-02损伤的拮抗作用。方法:观察5、10、25mg/L的LSPC与200mmol/L乙醇共同作用于肝细胞L-0224h后,对细胞的生存率,超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、丙二醛(MDA)含量、凋亡率以及DNA损伤等指标的影响。结果:与乙醇组相比,5mg/L和10mg/LLSPC使细胞的生存率由73.14%上升到84.79%和90.52%,提高了细胞SOD、CAT、GSH的含量(P<0.01),降低MDA的生成;有效降低细胞的凋亡率,降低细胞p53和生长抑制DNA损伤基因(GADD)34、45β、153的表达,但高剂量组(25mg/L)对乙醇的拮抗作用不明显。结论:适量浓度的莲房原花青素对乙醇诱导的肝细胞L-02的损伤有拮抗作用。

THE PROTECTIVE EFFECT OF LOTUS SEEDPOD ON HEPATOCYTE DAMAGE INDUCED BY ETHANOL

Objective: To study the protective effect of lotus seedpod procyanidins (LSPC) on ethanol-induced damage to human embryo liver L-02 cells. Method: L-02 cells were exposed to 200mmol/L ethanol, 200mmol/L ethanol + 5, 10 and 25 mg/L LSPC respectively for 24 h. Survival rate, levels of superoxide dismutase(SOD), glutathione (GSH), catalase (CAT), malonyldiadehyde (MDA), apoptosis rate and the expression level of DNA damage genes were measured. Results: Compared with the ethanol group, the L-02 cell survival rate of 5 and 10 mg/L LSPC group increased from 73.14% to 84.79% and 90.25% respectively, SOD, GSH, CAT levels increased , MDA level, apoptosis rate, the expression level of p53 and growth arrest and DNA damage-inducible genes (GADD) decreased . But the same effect wasn’t observed in 25 mg/L LSPC group. Conclusion: 5 mg/L and 10 mg/L LSPC can protect ethanol induced damage in human embryo liver L-02 cells.