| HPLC测定复方甘草片中甘草酸含量 |
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| 引用本文： | 李聪. HPLC测定复方甘草片中甘草酸含量[J]. 中国执业药师, 2012, 9(4): 17-19 |
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| 作者姓名： | 李聪 |
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| 作者单位： | 新农甘草产业有限责任公司,新疆,阿拉尔,843018 |
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| 摘 要： | 目的 建立应用高效液相色谱法测定复方甘草片中甘草酸含量的检测方法.方法 色谱柱Kromasil-Cl8柱,规格:4.6 mm × 150 mm,粒度:5 μm,柱温:25 ℃,流动相:乙腈-0.05%磷酸(36∶64),流速:1.0 mL/min,检测波长:250 nm,进样量:10 μL,以峰面积外标法定量.结果 甘草酸浓度在48.8 ~ 585.7 μg/mL有较好的线性范围,回归方程为Y = 560 4.4 X - 150 29(r = 0.999 9),平均回收率为100.1%(n = 6),RSD = 0.42%.结论 此方法适用于测定复方甘草片中甘草酸的含量.
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| 关 键 词： | 高效液相色谱法 复方甘草片 甘草酸 |
Content Determination of Glycyrrhizic Acid in Compound Liquorice Tablets by HPLC |
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| Li Cong. Content Determination of Glycyrrhizic Acid in Compound Liquorice Tablets by HPLC[J]. China Licensed Pharmasist, 2012, 9(4): 17-19 |
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| Authors: | Li Cong |
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| Affiliation: | Li Cong(Xinnong Licorice Industry Co.Ltd.,Xinjiang Alaer 843018,China) |
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| Abstract: | Objective：To establish a method for the content determination of glycyrrhizic acid in compound liquorice tablets by HPLC.Methods：The Kromasil-C18（4.6 mm × 150 mm） separation column was used with the column temperature at 25℃.The mobile phase was acetonitrile-0.05% phosphoric acid（36：64）,the flow rate was 1.0 mL/min,the UV detection wavelength was at 250 nm,and the sample volume was 10 μL.The external standard method of peak area was employed for quantitative analysis.Results：A good linearity was obtained in the range of 48.8 ～ 585.7 μg/mL,regression：Y = 560 4.4 X-150 29（r = 0.999 9）,the average recovery was 100.1%（n = 6,RSD = 0.42%）.Conclusion：This method is good for the content determination of glycyrrhizic acid in compound liquorice tablets. |
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| Keywords: | HPLC Compound Liquorice Tablets Glycyrrhizic Acid |
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