结核分枝杆菌感染对非小细胞肺癌患者调节性免疫细胞的负向调节作用

目的探讨结核分枝杆菌感染对非小细胞肺癌患者调节性免疫细胞的负向调节作用。 方法选择2014年3月到2018年3月温州市中心医院就诊的非小细胞肺癌合并结核分枝杆菌感染患者为感染组（10例），单纯非小细胞肺癌患者为肿瘤组（10例），同期选择10例健康体检者为对照组。采用免疫组织化学法检测Ki-67增殖水平；采用流式细胞术检测CD4⁺CD25⁺Foxp3⁺调节性T（Treg）细胞和CD19⁺IL-10⁺调节性B（Breg）细胞的比例。 结果肿瘤组Ki-67的比例为（35 ± 4）%，感染组为（66 ± 6）%，感染组Ki-67的比例显著高于肿瘤组（t = 4.068，P < 0.001）。三组肺癌合并结核患者外周血CD4⁺CD25⁺Foxp3⁺Treg细胞、CD19⁺IL-10⁺Breg细胞比例的比较，差异均有统计学意义（F = 69.400，P < 0.001；F = 16.090，P < 0.001）。且与肿瘤组CD4⁺CD25⁺Foxp3⁺Treg细胞比例（0.41 ± 0.03）%、（0.21 ± 0.03）%、（1.03 ± 0.08）%]、CD19⁺IL-10⁺Breg细胞比例（0.429 ± 0.021）%、（0.268 ± 0.013）%、（0.783 ± 0.111）%]比较，对照组均显著降低，而感染组均显著升高（P均< 0.05）。 结论非小细胞肺癌合并结核分枝杆菌感染患者外周血中Treg细胞和Breg细胞比例显著升高。

Negative regulation of Mycobacterium tuberculosis infection on regulatory immune cells in patients with non-small cell lung cancer

ObjectiveTo investigate the effect of Mycobacterium tuberculosis infection on regulatory immune cells in patients with non-small cell lung cancer (NSCLC). MethodsThe NSCLC patients in Wenzhou Central Hospital between March 2014 and March 2018 were selected as the infection group (n = 10) and cancer group (n = 10) according to whether suffering from Mycobacterium tuberculosis infection. Ten healthy subjects were enrolled as the control group at the same time. The proliferation level of Ki-67 was detected by immunohistochemistry. The flow cytometry was used to detect CD4⁺CD25⁺Foxp3⁺ regulatory T (Treg) cells and CD19⁺IL-10⁺ regulatory B (Breg) cells. ResultsThe proportion of Ki-67 was (35 ± 4)% in the cancer group and (66 ± 6)% in the infection group; it was much higher in the infection group than in the cancer group (t = 4.068, P < 0.001). The proportion of CD4⁺CD25⁺Foxp3⁺Treg cells and CD19⁺IL-10⁺Breg cells among three groups all showed significant differences (F = 69.400, P < 0.001; F = 16.090, P < 0.001). Compared with the cancer group, the proportion of CD4⁺CD25⁺Foxp3⁺Treg cells (0.41 ± 0.03)%, (0.21 ± 0.03)%, (1.03 ± 0.08)%] and CD19⁺IL-10⁺Breg cells(0.429 ± 0.021)%, (0.268 ± 0.013)%, (0.783 ± 0.111)%] decreased markedly in the control group, and increased obviously in the infection group (all P < 0.05). ConclusionThe proportion of Treg and Breg cells in peripheral blood of NSCLC patients with tuberculosis infection is significantly higher.