表面粗糙度对铸造纯钛表面细菌粘附影响的临床研究

目的 评价不同表面形态与表面粗糙度在对纯钛在口腔内戴用0．5年后的细菌粘附量与种类的影响。方法 常规制作纯钛铸造试件30个，分为皱纹形和不同粗糙度的平面形共5组。选择6名口腔粘膜健康的无牙颌受试者，在其上颌总义齿基托磨光面和组织面的不同部位粘固试件。戴用义6个月后，检测各组试件粘附细菌的情况。结果 ①患者个体因素对纯钛铸件表面粘附的细菌量明显的影响，但对细菌地染色分类影响不明显。②同一患者，试件表面粗糙度增加，细菌量明显增大（P＜0．01），但细菌组成无明显变化；皱纹形试件比平面试件滞留的细菌数量多，除G^＋球菌外，还附着G^-球；相同粗糙度的2个试件，放于组织面者滞留的细菌数量比磨光面者明显减少（P＜0．01），且前者粘附的主要为G ^-球菌和杆菌。结论 从有益牙周组织健康的角度看，制作纯钛修复体时应可能采用平面形钛制修复体，磨改后应重视抛光，尤其是组织面。

Influence of surface roughness of pure Titanium on accumulation of bacterium

OBJECTIVE: The aim of this investigation was to evaluate the influence of surface roughness of pure titanium on adhesion of bacterium. METHODS: A total of six edentulous volunteers with healthy oral mucosa participated in an in vivo study. Four kinds of pure Titanium testing pieces with different surfaces were fixed in the polished surface of upper complete dentures and the other in the tissue surface of the dentures. After 6-month wearing the dentures, the amount and species of bacterium adhered on pure Titanium were examined. RESULTS: (1) Individual difference had a significant influence on amount of bacterium adhered on pure Titanium, but had no relation to species of bacterium according to Gram's staining. (2) To the same patient, with the increase of roughness, bacterium adhered on samples improved in amount (P < 0.01), but remained the same in composition. Wrinkly samples collected more bacterium than plane samples and exhibited G- coccus beside G+ coccus. Comparing two samples with same roughness, the one with roughness on the tissue surface of the denture collected less bacterium than that on the polished surface (P < 0.01), and predominantly presented G- rod bacterium and coccus, which was completely different to that on the polished surface. CONCLUSION: From the perspective of benefit to periodontal tissue, plane surface should be adopted when framework of pure Titanium is made, and polishing of the prosthesis after being modified should be paid more attention, especially on the tissue surface.