淫羊藿提取物的质量研究

目的：考察目前市场上淫羊藿提取物的质量。方法：采用HPLC测定市售的淫羊藿提取物中朝藿定C、淫羊藿苷、箭藿苷B的含量和指纹图谱,紫外分光光度法测定总黄酮含量。结果：大部分提取物中淫羊藿苷含量与公司提供的标示量一致,全部测定样品的指纹图谱分为3类,分别对应于不同的来源药材,淫羊藿苷标示含量相同的样品中朝藿定C、箭藿苷B以及总黄酮的含量存在差异。结论：将提取物指纹图谱与药材指纹图谱进行比对,可以初步确定其来源的药材,药材的不同是造成提取物质量差异的主要因素,建议淫羊藿提取物应该标示药材来源,并进行多项的含量检测,以便实现提取物质量的稳定。

Research on quality of Epimedium extract in market

Objective:To review the quality of Epimedium extract in the market.Method:The contents of icariin,epimedin C, sagittatoside B and total flavonoids in Epimedium extracts sold in the market were assayed by the methods of HPLC and UV respective- ly.HPLC fingerprintings were obtained at the same time.Result:The contents of icariin in most of the extracts are closely similar with the ones labeled by the companies.3 type chromatograms were classified in all the HPLC fingerprintings,and were corresponded with their raw materials.The contents of epimedin C,sagittatoside B and total flavonoids were different in the samples with the same content of icariin.Conclusion:We can primarily confirm the origin of raw materials by comparing the HPLC fingerprinting of extracts with the ones of materials.The difference of extracts quality mainly comes from the difference of materials.So we suggest that Epimedium extract product should be labeled the origin of materials,and assayed with more compound contents,to ensure the quality stabilization.