AM及其抑制剂对人卵巢癌细胞株(CAOV3)PKB活性的影响

目的探讨肾上腺髓质素(Adrenom edullin,AM)及AM受体阻断剂(AM22-52)对人卵巢癌细胞株CAOV3蛋白激酶B(PKB)活性的影响。方法以不同浓度的AM和AM22-52诱导CAOV3细胞,利用Western印迹方法,测定PKB活性的变化。结果AM和AM受体阻断剂AM22-52对t-PKB(total-PKB)的表达量均无明显影响;随着AM浓度(1×10-9～1×10-6mol/L)的增加,CAOV3细胞p-PKB活性随之升高,呈剂量依赖关系;AM受体阻断剂AM22-52(1×10-9～1×10-6mol/L)抑制CAOV3细胞内p-PKB活性,也呈剂量依赖效应。结论AM激活CAOV3细胞内的PKB信号通路,AM22-52可有效阻滞此传导途径,为卵巢癌的生物治疗提供新的思路。

Effects of AM and AM22-52 on Activity of PKB in Human Ovarian Epithelial Cancer Cells Line CAOV3

Objective To explore the effects of adrenomedullin （AM） and AM receptor inhibitor （AM22-52） on the activity of the protein kinase B （PKB） in human ovarian epithelial cancer cell line CAOV3. Methods The activity of PKB in CAOV3 cells induced by different concentration of AM and AM22-52 was detected by Western blot. Results There was no obvious difference for the expression of total-PKB （t-PKB） between control group and experiment groups （AM group or AM22-52 group）. AM （1 × 10^-9 - 1 × 10^-6mol/L） could improve the activity of p-PKB in CAOV3 significantly, AM22-52 （1× 10^-9～ 1 × 10^-6mol/L） could suppress the activity of p-PKB, and both with a dose-dependent manner （ P 〈0.05）. Conclusion The results of activation of PKB signal pathway by AM in CAOV3 and inhibition of the signal pathway by AM22-52 might provide a new route for the treatment of ovarian cancer.