硒酸酯多糖与紫杉醇联合应用对肝癌HepG-2细胞生长的抑制作用

目的体外观察硒酸酯多糖(KSC)与紫杉醇(Taxol)对肝癌细胞HepG-2生长的协同抑制效应,并探讨其作用机制。方法 MTT法观察KSC和Taxol单用及合用对HepG-2细胞的抑制作用,并计算两药合用72 h的IC50及合用指数CDI;采用荧光显微镜、流式细胞仪观察上述药物作用后HepG-2细胞形态学、细胞周期及凋亡率的改变。结果MTT结果显示,KSC与Taxol均能显著抑制HepG-2细胞的增殖,且具有时效-量效关系,联合组效果优于单一用药组;KSC、Taxol单用72 h的IC50分别为46.632 mg.L-1和1.684 nmol.L-1,两药联合应用后的IC50分别降至31.684 mg.L-1和0.272 nmol.L-1。30 mg.L-1的KSC与0.5~5 nmol.L-1的Taxol联合效果表现为协同作用(CDI<1.0)。细胞形态学观察可见细胞数减少,细胞核明显变小,并可见致密强荧光及凋亡小体。流式细胞术结果显示两药均作用于S期,联合组既可发生S期周期阻滞,又可诱导凋亡,但凋亡率低于Taxol单独组。结论 KSC与化疗药物Taxol联合可产生协同作用,诱导肿瘤细胞周期阻滞可能为其主要...

Growth inhibition of kappa-selenocarrageenan in combination with paclitaxol on human hepatoma HepG-2 cell line

Objective To investigate the effect and its possible mechanism of combined use of kappa-selenocarrageenan(KSC) with paclitaxol(Taxol) on HepG-2 hepatoma cells in vitro.Methods MTT assay was used to estimate the growth inhibition of HepG-2 cells by using KSC,Taxol alone or the both.The IC50 and combination index(CDI) after 72 h of using two drugs separately or together were calculated.The cell morphology was observed by fluorescent microscopy.Cell-cycle and apoptotic rates were analyzed by flow cytometry.Res...