| 海洋链霉菌Streptomyces sp.SCSIO 1666活性代谢产物替达霉素A和B的发酵优化及分离鉴定 |
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| 引用本文： | 段传人,姚月良,汪中文,田新朋,张偲,张长生,鞠建华.海洋链霉菌Streptomyces sp.SCSIO 1666活性代谢产物替达霉素A和B的发酵优化及分离鉴定[J].中国海洋药物,2010,29(6):12-20. |
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| 作者姓名： | 段传人 姚月良 汪中文 田新朋 张偲 张长生 鞠建华 |
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| 作者单位： | 重庆大学生物工程学院;中国科学院海洋生物资源可持续利用重点实验室;广东省海洋药物重点实验室;中国科学院海洋微生物中心中国科学院南海海洋研究所; |
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| 基金项目： | 中国科学院知识创新工程重要方向项目(KSCX2-YW-G-065,KZCX2-YW-JC202,KSCX2-YW-G-073); 中国科学院南海海洋所领域前沿项目(LYQY200805) |
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| 摘 要： | 目的对南海海洋沉积物中分离的海洋放线菌进行体外抗肿瘤细胞和抗菌模型筛选,选取生物活性强的海洋链霉菌菌株Streptomyces sp.SCSIO1666进行发酵条件优化和活性产物的分离及结构鉴定。方法用针对6种NCI肿瘤细胞株A549、DU145、H1299、HCT15、HEP3B和SF268的体外抗肿瘤模型及其抑菌模型对提取物进行筛选,进一步优化发酵条件,采用HPLC-UV和枯草芽孢杆菌(Bacillussubtilis)抑菌活性追踪,利用有机溶剂萃取、正相硅胶和反相硅胶等各种色谱层析分离,通过理化性质和波谱数据分析进行结构鉴定。结果与结论从一株海洋链霉菌菌株Streptomyces sp.SCSIO 1666发酵产物中分离得到替达霉素A和B(TirandamycinsA and B),替达霉素A和B的生产对海盐的依赖程度较高,在不加盐的条件下产量极低,加3%粗海盐时,其发酵产量提高250倍以上,说明盐胁迫是海洋链霉菌菌株SCSIO1666产生活性物质的重要条件。替达霉素B是终产物,加入2%大孔吸附树脂发酵时,替达霉素A的产量得到明显提高。
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| 关 键 词： | 海洋放线菌 Streptomyces sp.SCSIO 1666 替达霉素A和B 发酵优化 抗肿瘤 抗菌 |
Fermentation optimization,isolation and identification of tirandamycins A and B from marine-derived Streptomyces sp.SCSIO 1666 |
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| DUAN Chuan-ren,YAO Yue-liang,WANG Zhong-wen,TIAN Xin-peng.Fermentation optimization,isolation and identification of tirandamycins A and B from marine-derived Streptomyces sp.SCSIO 1666[J].Chinese Journal of Marine Drugs,2010,29(6):12-20. |
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| Authors: | DUAN Chuan-ren YAO Yue-liang WANG Zhong-wen TIAN Xin-peng |
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| Institution: | DUAN Chuan-ren~1,YAO Yue-liang~1,WANG Zhong-wen~(2,3,4),TIAN Xin-peng~(2,ZHANG Si~(2,ZHANG Chang-sheng~(2,JU Jian-hua~(2,4*) (1.Bioengineering College,Chongqing University,Chongqing 400030,China,2.Key Laboratory of Marine Bio-resourses Sustainable Utilization,3.Guangdong Key Laboratory of Marine Materia Medica,4.RNAM Center for Marine Microbiology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China) |
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| Abstract: | Objective To screen antitumor and antibacterial agents from marine actinomycetes isolated from marine sediment,and strain Streptomyces sp.SCSI01666 was selected for further fermentation optimization to produce bioactive secondary metabolites.Methods Bioassay was conducted using in vitro antitumor activities against six tumor cell lines A549,DU145,H1299,HCT15,HEP3B,SF268,and using antibacterial models.The fermentation conditions of the strain were optimized and the bioactive secondary metabolites were isolat... |
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| Keywords: | marine actinomycetes streptomyces sp Scsiol666 tirandamycins A and B fermentation optimization antitumor antibacterial |
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