Changes in the lectin binding sites on the testicular, epididymal, vas, and ejaculated spermatozoon surface of dog |
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Authors: | H. K. Bains M. A. Pabst and Professor Dr. S. R. Bawa |
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Affiliation: | Department of Biophysics, Panjab University, Chandigarh-160 014, India;Department of Histology and Embryology, University of Graz, Harrachgasse 21, A 8010 Graz, Austria |
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Abstract: | Summary. Changes in the localization of sperm surface glycocomponents of testicular, epididymal, vas deferens, and ejaculated spermatozoa of dog ( Canis domesticus ) were studied employing fluorescein isothiocyanate conjugated lectins viz., Concanavalin A (ConA), Triticum vulgaris (WGA), Maclura pomifera (MPA), and Arachis hypogaea (PNA) agglutinins. The plasma membrane clothing the acrosome of the testicular, epididymal, and vas deferens spermatozoa shows reactivity with all the lectins used. However, in the ejaculated spermatozoa, the entire sperm surface shows reactive sites for ConA, WGA, and PNA. Variation in the labelling of the cytoplasmic droplet in different stages of spermatozoon transit in the epididymis has been discussed. |
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Keywords: | Spermatozoa lectins fluorescence microscopy dog. |
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