乙酰半胱氨酸镁盐-甘草酸环状样二聚体治疗大鼠肝纤维化的实验研究

目的：研究新合成产物乙酰半胱氨酸镁盐-甘草酸环状样二聚体（acetylcysteine magnesiumglycyrrhizin fine antipode dimers,GFA-NAC·Mg）治疗大鼠肝纤维化的效果.方法：25％四氯化碳（CC14）制备大鼠肝纤维化模型,GFA-NAC·Mg按25、50、100mg/kg剂量每日分别腹腔注射进行肝纤维化治疗,给药8周后处死大鼠,用肝纤维化网格染色观察肝纤维化情况,ELISA法测定血清羟脯氨酸（Hyp）和肝组织丙二醛（MDA）、超氧化物歧化酶（SOD）、转化生长因子-β1（TGF-β1）、血小板衍生生长因子（PDGF）.结果：大鼠肝组织病理结果显示其肝纤维化处于Ⅲ-Ⅳ期;与模型组比较,GFA-NAC· Mg治疗后血清Hyp明显降低;肝组织抗氧化指标SOD显著升高,MDA明显下降;肝纤维化指标TGF-β1、PDGF在组织含量下降;GFA-NAC·Mg各组网格染色显示肝纤维化好转.结论：GFA-NAC·Mg对肝纤维化大鼠有较好实验治疗作用,可能通过抑制PDGF和TGF-β1的表达而实现.

Experimental study of acetylcysteine magnesium-glycyrrhizin fine antipode dimers on rat liver fibrosis

AIM：To investigate efficacy of GFA-NAC · Mg on rat liver fibrosis.METHODS：Preparation of liver fibrosis model in rats with 25% carbontetrachloride （CCl4） by subcutaneous injection,GFA-NAC · Mg intraperitoneal injected treating liver fibrosis with 25,50 and 100 mg/kg.All the rats were killed after 8 weeks of administration with grid staining and the levels of Hyp in serum were detected with ELISA assay,the contents of the MDA,SOD,TGF-β1,PDGF for liver fibrosis and liver tissue were tested.RESULTS：The liver tissue of fibrosis showed stage Ⅲ-Ⅳ.Compared with the model group,the serum of Hyp significantly was reduced after GFA-NAC · Mg treatment.The antioxidant index SOD significantly was increased in liver tissue while MDA was decreased.Liver fibrosis markers TGF-β1 and PDGF content were decreased.Liver fibrosis was improved after GFA-NAC· Mg treatment in each groups by grid staining.CONCLUSION：GFA-NAC · Mg has a better therapeutic effect on rat liver fibrosis by inhibiting the expression of PDGF and TGF-β1.