返魂草干粉吸入剂的含量测定及体外肺沉积率考察

目的 建立高效液相色谱法测定返魂草干粉吸入剂中返魂草素Ⅱ含量的方法,并对其体外沉积性质进行初步考察.方法 采用Eclipse XDB-C18柱（4.6 mm×150 mm,5μm）,流动相为CH3OH-5 mmol/L NaH2PO4配制的6 mmol/L的四丁基溴化铵（2 mol/L的NaOH调pH 7.0）(30:...

Content Determination and in Vitro Lung Deposition Properties of Senecio Cannabifolius Less.DPIs

Objective To establish an high performance liquid chromatography method for determination of FanhuncaoinⅡin Senecio cannabifolius Less.DPIs,and investigate its deposition property in vitro.Methods An Eclipse XDB-C18 column（4.6 mm×150 mm,5 μm） was used with a mixture of methanol-buffer solution（5 mmol/L sodium dihydrogen phosphate and 6 mmol/L tetrabutylammonium bromide,adjusted to pH 7.0 with 2 mol/L sodium hydroxide）（30∶70） as the mobile phase.The flow rate was 1.0 ml/min and the detection wavelength was set at 230 nm.The in vitro deposition properties of the powder were assessed with a twin stage impinge.Results The linear range of FanhuncaoinⅡwas 5-150 μg/ml（r=0.9994）.The average recovery was 97.2%,and RSD was 1.4%（n=5）.The in vitro deposition test showed that the lung deposition rate was more than 10%.Conclusion This method is credible,reproducible and suitable for determination of Senecio cannabifolius Less.DPIs.The in vitro lung deposition rate meets the standard.