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| 高效液相色谱法测定金栀洁龈含漱液盐酸小檗碱的含量 | |
| 引用本文： | 李显峰,毕雪艳,李晓菊,薛卫艳,王志洁.高效液相色谱法测定金栀洁龈含漱液盐酸小檗碱的含量[J].解放军药学学报,2011,27(4):344-345. |
| 作者姓名： | 李显峰 毕雪艳 李晓菊 薛卫艳 王志洁 |
| 作者单位： | 1. 河北省邯郸市眼科医院(邯郸市第三医院), 河北邯郸,056001 2. 河北省邯郸药品检验所, 河北邯郸,056001 3. 河北省邯郸市妇幼保健院, 河北邯郸,056001 4. 河北省人民医院, 河北石家庄,050051 |
| 摘 要： | 目的 采用高效液相色谱法测定金栀洁龈含漱液中盐酸小檗碱的含量.方法 以AGILENT C18为色谱柱,乙腈-0.3％磷酸溶液(42:58)(每100 ml加十二烷基磺酸钠0.1g)为流动相,以265 nm为检测波长,流速为1.0 ml/min.结果 盐酸小檗碱在5～50 μg/ml范围内线性关系良好,r=0.9999,... |
| 关 键 词： | 金栀洁龈含漱液 盐酸小檗碱 高效液相色谱法 |
High Performance Liquid Chromatography Determination of the Content of Berberine hydrochloride in Jinzhijieyinhanshuye |
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| LI Xian-feng,BI Xue-yan,LI Xiao-ju,XUE Wei-yan,WANG Zhi-jie.High Performance Liquid Chromatography Determination of the Content of Berberine hydrochloride in Jinzhijieyinhanshuye[J].Pharmaceutical Journal of Chinese People's Liberation Army,2011,27(4):344-345. | |
| Authors: | LI Xian-feng BI Xue-yan LI Xiao-ju XUE Wei-yan WANG Zhi-jie |
| Institution: | ④①Handan Eye Hospital of Hebei Province(Handan 3rd Hospital),Handan056001,China;②Handan Institute for Drug Control,Handan056001,China;③Maternity and Children Healthcare Hospital of Handan,Handan056001,China;④Hebei Provincial Peoples Hospital,Shijiazhuang050051,China |
| Abstract: | Objective High performance liquid chromatography method was developed to determine the content of berberine hydrochloride in Jinzhijieyinhanshuye.Methods It was determined on an AGILENT C18 column,using Acetonitrile-0.3%phosphoric acid solution（42∶58）（the 100 ml solution containing 0.1 g sodium dodecyl sulphate）as the mobile phase at a flow rate of 1.0 ml/min,and detected at 265 nm.Results The linear relation of the peak area of berberine hydrochloride（5-50 μg/ml） was determined by high performance liquid chromatography with a regression coefficient of 0.9999 and the average recovery rate was 98.81%,RSD was 0.4%.Conclusion The method is simple,quick and accurate.It can be used for determining Jinzhijieyinhanshuye. |
| Keywords: | Jinzhijieyinhanshuye berberine hydrochloride high performance liquid chromatography |
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