| 骨唾液酸蛋白对乳腺癌细胞MCF-7侵袭转移能力的影响 |
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| 引用本文： | 张洁,常海敏,赵杰,朱慧芳,韩芳毅,千新来.骨唾液酸蛋白对乳腺癌细胞MCF-7侵袭转移能力的影响[J].新乡医学院学报,2008,25(3):222-225. |
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| 作者姓名： | 张洁 常海敏 赵杰 朱慧芳 韩芳毅 千新来 |
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| 作者单位： | 1. 新乡医学院病理学教研室,河南,新乡,453003
2. 新乡医学院法医临床学教研室,河南,新乡,453003 |
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| 基金项目： | 河南省高校杰出科研创新人才工程项目 |
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| 摘 要： | 目的研究骨唾液酸蛋白（BSP）对乳腺癌细胞MCF-7侵袭转移能力的影响及机制。方法通过划痕试验、Transwell侵袭实验、细胞黏附实验和反转录-聚合酶链反应等观察BSP对乳腺癌细胞MCF-7侵袭转移能力的影响。结果BSP稳定高表达可使MCF-7细胞的侵袭能力明显增加。稳定高表达BSP的MCF-7-BSP细胞与胶原和纤维连接蛋白的黏附性增加,其基质金属蛋白酶（MMP）-2和MMP-9表达也增强。结论BSP可以促进MCF-7细胞侵袭转移能力,其可能的机制是通过增加细胞的黏附性、促进MMP-2和uPA表达等。
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| 关 键 词： | 骨唾液酸蛋白 乳腺癌 侵袭 转移 |
| 文章编号： | 1004-7239(2008)03-0222-04 |
| 修稿时间： | 2008年3月19日 |
Influences of bone sialoprotein on the invasive and metastatic abilities of MCF-7 cells of breast carcinoma |
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| ZHANG Jie,CHANG Hai-min,ZHAO Jie,et al.Influences of bone sialoprotein on the invasive and metastatic abilities of MCF-7 cells of breast carcinoma[J].Journal of Xinxiang Medical College,2008,25(3):222-225. |
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| Authors: | ZHANG Jie CHANG Hai-min ZHAO Jie |
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| Institution: | ZHANG Jie1,CHANG Hai-min2,ZHAO Jie1,et al |
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| Abstract: | Objective To investigate the influences of bone sialoprotein(BSP) on the invasive and metastatic abilities of MCF-7 cells and its mechanisms.Methods The influences of BSP on the invasive and metastatic abilities of MCF-7 cells and its mechanisms were investigated by scarification test,transwell invasion assay,cell adhesion assay,and RT-PCR.Results BSP overexpression could promote the invasive and metastatic abilities of MCF-7 cells.The MCF-7-BSP cells of BSP stable overexpression increased the adhesiveness with collagen and fibronectin,and the expression of MMP-2 and MMP-9 was also increased.Conclusion BSP can promote the invasive and metastatic abilities of MCF-7 cells.These biological function maybe correlate to its abilities of increasing the adhesiveness of the cells,and promoting the expression of MMP-2 and MMP-9. |
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| Keywords: | bone sialoprotein breast cancer invasion metastasis |
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