EBV4型IgA/VCA IgA/EA IgG/EA IgG/ZEBRA抗体在鼻咽癌普查和早期诊断中的应用

目的摸索以疱疹病毒4型（EBV）IgG／ZEBRA为捕捉抗原的间接酶联免疫吸附试验（ELISA）条件,为大量人群普查奠定基础。方法将纯化的ZEBRA抗原用于对鼻咽癌（NPC）患者血清及健康人血清IgG／ZEBRA抗体的ELISA检测。结果检测NPC患者血清288份,其中ELISA实验显示阳性262份,敏感度91％,检测正常人血清96份,其中阳性5份,特异度94．8％。其结果显示NPC组的阳性率与健康对照组的数据之间差异有统计学意义（P〈0．001）。本研究在此基础上对广东惠州5463份和广西桂平2017份血清进行检测,检出早期鼻咽癌患者5例。并将结果与免疫酶法检测IgA／VCA、IgA/EA、IgG／EA比较。结论以EBV早期抗原ZEBRA为捕捉抗原的间接ELISA方法具有较高的特异性和敏感性,可以用于大量人群的NPC早期筛查和早期诊断。

Comparison of IgA/VCA, IgA/EA, IgG/EA in immunoenzyme methods and ZEBRA ELISA in early diagnosis of nasopharyngeal carcinoma

Objective To develop an ELISA method using Herpesvirus hominis type 4(EBV) IgG/Zebra as capture antigen for large population screening.Methods The ELISA method used purified ZEBRA antigen to detect the IgG/ZEBRA antibody from serum in nasopharyngeal carcinoma (NPC) and normal healthy subjects.Results Of 288 NPC sera,262 were detected positive,the sensitivity was 91%,while 5 of 96 normal sera were detected positive,the specificity was 94.8% and the results of NPC group and healthy group displayed significant difference(P<0.001). IgA/VCA,IgA/EA,IgG/EA in immunoenzyme methods and ZEBRA ELISA were compared during the NPC screening in two cities: Huizhou,Guangdong and Guiping,Guangxi, 5463 and 2017 samples respectively were tested and 5 earlier NPC patients were found. Conclusion The results indicate that this method has high specificity and sensitivity,and can be used for large population screening to assist early phase NPC diagnosis.