慢性乙型肝炎的临床和分子诊断研究 |
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引用本文: | 许国胜,陈慧,黄雪玲,甄英鹏,郭菁,陆伟桃.慢性乙型肝炎的临床和分子诊断研究[J].齐齐哈尔医学院学报,2012,33(16):2201-2203. |
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作者姓名: | 许国胜 陈慧 黄雪玲 甄英鹏 郭菁 陆伟桃 |
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作者单位: | 1. 广州市萝岗区永和街社区卫生服务中心检验科 510730 2. 广州市开发区医院功能科 3. 广州市黄埔区红十字会医院检验科 |
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基金项目: | 广州市黄埔区科学技术局科研课题 |
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摘 要: | 目的 以分子诊断技术手段,探讨慢性乙型肝炎(CHB)患者血清病毒复制指标(HBV-DNA)与乙肝标志物(HBV- M)及肝功能关系.方法 运用荧光定量PCR(FQ-PCR)检测乙肝患者血清中HBV- DNA,同时运用ELISA方法进行两对半指标的检测,用全自动生化仪检测肝功能,并对结果进行相关性探讨.结果 小三阳组HBV- DNA阳性率为52.2%,大三阳组HBV- DNA阳性率为96.1%,小三阳组与大三阳组,与3组,与5组相比差异有显著性(P<0.05); ALT和HBV- DNA有显著差异性(P<0.05),并将本地区慢性乙型肝炎人群分为A、B、C、D四型.结论 本地区慢性乙型肝炎患者以B型占多数,小三阳病人HBV仍然处于活动期,其体内病毒复制并没有停止或消失,ALT和HBV- DNA没有必然的相关性.
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关 键 词: | 慢性乙型肝炎 HBV血清标志物 HBV-DNA FQ-PCR ALT |
Research on chronic B type hepatitis clinical and molecular diagnosis |
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Institution: | XU Guo-sheng,et al.(Clinical Laboratory Section,Yonghe Street Community Health Service Center,Guangzhou,Guangdong 510730,the P.R.China.) |
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Abstract: | Objective At the molecular diagnosis technology,this paper discusses the chronic hepatitis b(CHB) patients serum virus replication index(HBV-DNA) and hepatitis b markers(HBV-M) and liver function relations. Methods Using fluorescence quantitative PCR(FQ-PCR) testing hepatitis b patients serum HBV-DNA,and using ELISA method of two half-and-half index test,with automatic biochemistry analyzer test liver function,and the results were correlation discussion. Results The small HBV-DNA positive rate was 52.2%,the big HBV-DNA positive rate was 96.1%(P<0.05);ALT and HBV-DNA significant difference(P<0.05),and chronic hepatitis B in the crowd into A,B,C,D four type. Conclusions The region in patients with chronic hepatitis B type B in the majority,small HBV is still in active period,the virus copy inside its body don’t stop or disappear,there is no necessary relevance between ALT and HBV-DNA. |
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Keywords: | Chronic hepatitis b HBV serum markers HBV-DNA FQ-PCR ALT |
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