慢性乙型肝炎的临床和分子诊断研究

目的 以分子诊断技术手段,探讨慢性乙型肝炎(CHB)患者血清病毒复制指标(HBV-DNA)与乙肝标志物(HBV- M)及肝功能关系.方法 运用荧光定量PCR(FQ-PCR)检测乙肝患者血清中HBV- DNA,同时运用ELISA方法进行两对半指标的检测,用全自动生化仪检测肝功能,并对结果进行相关性探讨.结果 小三阳组HBV- DNA阳性率为52.2％,大三阳组HBV- DNA阳性率为96.1％,小三阳组与大三阳组,与3组,与5组相比差异有显著性(P＜0.05); ALT和HBV- DNA有显著差异性(P＜0.05),并将本地区慢性乙型肝炎人群分为A、B、C、D四型.结论 本地区慢性乙型肝炎患者以B型占多数,小三阳病人HBV仍然处于活动期,其体内病毒复制并没有停止或消失,ALT和HBV- DNA没有必然的相关性.

Research on chronic B type hepatitis clinical and molecular diagnosis

Objective At the molecular diagnosis technology,this paper discusses the chronic hepatitis b(CHB) patients serum virus replication index(HBV-DNA) and hepatitis b markers(HBV-M) and liver function relations. Methods Using fluorescence quantitative PCR(FQ-PCR) testing hepatitis b patients serum HBV-DNA,and using ELISA method of two half-and-half index test,with automatic biochemistry analyzer test liver function,and the results were correlation discussion. Results The small HBV-DNA positive rate was 52.2%,the big HBV-DNA positive rate was 96.1%(P<0.05);ALT and HBV-DNA significant difference(P<0.05),and chronic hepatitis B in the crowd into A,B,C,D four type. Conclusions The region in patients with chronic hepatitis B type B in the majority,small HBV is still in active period,the virus copy inside its body don’t stop or disappear,there is no necessary relevance between ALT and HBV-DNA.