Role of Extracellular Matrix in Acupoint Region in Acupuncture Effect

目的：探讨穴区细胞外基质在针刺信号转导中的作用机制。方法：SD大鼠40只，雌雄各半，随机分为4组，每组10只，以大鼠热辐射甩尾实验的温度变化为观察指标。实验组大鼠“后三里”区注射人工合成的五肽甘氨酰-精氨酰-甘氨酰-天冬酰-酪氨酸（Gly—Arg-Gly-Asp-Tyr,GRGDY）30min后再针刺，以大鼠基础痛阈组、“后三里”区注射0．9％NaCl组及针刺后三里组为对照。结果：GRGDY注射后30min再针刺，大鼠甩尾的温度与针刺组相比未见明显改变。结论：GRGDY未能阻断针刺效应，针刺效应的初始机制可能与细胞外基质与整合素特异性的结合位点RGD无关。

Role of extracellular matrix in acupoint region in acupuncture effect

Objective: To investigate the action mechanism of extracellular matrix in acupoint region in acupuncture signal transmission. Methods: Forty SD rats, half male and half female, were randomly allocated to the experiment group, basic pain threshold group, normal saline group and acupuncture group, with 10 rats in each group, the tail temperature at which rat flicks its tail in heat radiation tail flick test was taken as an observation index. In experiment group, the rats were given acupuncture treatment 30 min after injection of synthetic pentapeptide GRGDY (glycyl-arginyl-glycyl-aspartyl-tyrosine, Gly-Arg-Aly-Asp-Tyr) into “Housanli” area, basal pain threshold group, normal saline group which was given injection of 0.9% NaCl into “Housanli” area and acupuncture group which was given acupuncture at “Housanli” were used as controls. Results: As compared with acupuncture group, the temperature at which rat flicked its tail did not significantly changes following acupuncture 30 min after injection of GRGDY. Conclusion: GRGDY could not prevent the acupuncture effect, and the initial mechanism of acupuncture effect might have no relation to the structural site that extracellular matrix combines with integrin specially Foundation project: Financially supported by Shanghai development fund of science and technology (04DZ19839) and national key basic research development plan (2005CB523306)