内源性白介素-10在心肌缺血-再灌注损伤中的作用

目的　探讨大鼠心肌缺血 再灌注过程中血清白介素 10 (IL 10 )浓度的变化 ,以及甲泼尼龙对内源性IL 10产生的影响。方法　将 6 3只大鼠随机分成假手术组、缺血 再灌注 (对照 )组、甲泼尼龙(药物 )组。分别测定缺血 0 5h、再灌注 0 5h及 2h时血清中IL 10、磷酸肌酸激酶同功酶 (CK MB)的含量和心肌梗死面积。结果　对照组与药物组IL 10和CK MB含量自缺血 0 5h、再灌注 0 5h至 2h均呈逐渐升高趋势 ,再灌注 2h与再灌注前相比具有统计学差异 (P <0 0 5 )。再灌注后相应时段内 ,药物组较对照组IL 10明显升高 (P <0 0 5 ) ,心肌梗死面积减少 (P <0 0 5 ) ,CK MB降低且升高延迟。结论　在大鼠心肌缺血 再灌注中 ,甲泼尼龙可促进内源性IL 10大量释放。IL 10通过减轻心肌缺血 再灌注损伤发挥保护作用

Effects of interleukin-10 on myocardial ischemia and reperfusion injury in rats

Objective To investigate the effects of interleukin-10(IL-10) on myocardial ischemia and reperfusion (I/R) in rats. Methods Sixty-three rats were randomly divided into three groups, including sham group, I/R (control) group and Methylprednisolone-treated (administration) group. Serum IL-10 level and creatine kinase isoenzyme MB (CK-MB) concentration were measured,and myocardial infarct size was assessed at 0.5 hour after ischemia and 0.5 and 2 hours after reperfusion.Results In control and administration groups, serum IL-10 level and CK-MB concentration increased gradually from 0.5 hours after ischemia to 2 hours after reperfusion and increased significantly at 2 hours after reperfusion (P<0.05). IL-10 level in administration group was markedly higher than that in control group at the same time points after reperfusion. In administration group, CK-MB concentration and myocardial infarct size were significantly lower than those in the control group after reperfusion,while the increase of CK-MB was delayed.Conclusion This indicates that Methylprednisolone promotes highly release of endogenous IL-10,which has myocardial protective effect by inhibiting inflammatory response during I/R.