柴地合方对慢性应激大鼠大脑前额皮质和海马单胺类神经递质的影响

目的：研究柴地合方对脑组织单胺递质含量的影响，探讨其抗抑郁作用可能的机制。方法：复制大鼠慢性应激抑郁模型，慢性给予柴地合方，采用高效液相色谱系统(HPLC)加电化学检测器(ECD)检测前额皮质和海马组织中单胺类神经递质及相关代谢产物的含量。结果：慢性应激大鼠前额皮质中去甲肾上腺素(NE)、5羟色胺(5—HT)和3，4—二羟基苯乙酸(DOPAC)含量显著降低，柴地合方可以翻转这种降低；慢性应激大鼠海马中NE、5—HT含量显著降低，柴地合方可以部分逆转降低水平。结论：柴地合方可升高慢性应激模型大鼠前额皮质和海马中NE和5—HT水平，这可能是其抗抑郁的机制之一。

Effect of Chai Di Hefang on Monoamine Neurotransmitters in the Prefrontal Cortex and Hippocampus of Chronic Stress Rats

Objective: To explore the effect of Chai Di Hefang on monoamine neurotransmitters for probing into its antidepressant mechanism. Method: The rat chronic stress depression model was set up and Chai Di Hefang was chronically administered. The levels of monoamine neurotransmitters and their metabolites detected by HLPC-ECD. Result: The levels of NE, 5-HT and DOPAC in the prefrontal cortex of chronic stress rats decreased , and Chai Di Hefang could reverse them.The levels of NE and 5-HT in the hippocampus of chronic stress rats decreased , and Chai Di Hefang could reverse them partly. Conclusion: Chai Di Hefang can increase the levels of NE and 5-HT in the prefrontal cortex and hippocampus of chronic stress rats. This may be its part antidepressant mechanism.