BODIPY-conjugated chitosan nanoparticles as a fluorescent probe |
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| Authors: | Gizem Bor Muhammed Üçüncü Mustafa Emrullahoğlu Aysel Tomak |
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| Affiliation: | 1. Biotechnology and Bioengineering Department,;2. Chemistry Department and;3. Materials Science and Engineering Department, Izmir Institute of Technology, Izmir, Turkey |
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| Abstract: | Recently, development of fluorescent nanoparticle-based probes for various bioimaging applications has attracted great attention. This work aims to develop a new type fluorescent nanoparticle conjugate and evaluate its cytotoxic effects on A549 and BEAS 2B cell lines. Throughout the study, ionically crosslinked chitosan nanoparticles (CNs) were conjugated with carboxylated 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY-COOH). The results of conjugates (BODIPY-CNs) were investigated with regard to their physic-chemical, optical, cytotoxic properties and cellular internalization. The morphology of BODIPY-CNs was found to be spherical in shape and quite uniform having average diameter of 70.25?±?11.99?nm. Cytotoxicty studies indicated that although BODIPY-COOH itself was quite toxic on both A549- and BEAS 2B-treated cells, CNs increased the cell viability of both cell lines via conjugation to BODIPY-COOH fluorescent molecule up to 67% for A549 and 74% for BEAS 2B cells. These results may suggest a possible utilization of the new fluorescent nanoparticle-based probe for bioimaging in biology and medicine. |
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| Keywords: | Chitosan nanoparticles BODIPY cytotoxicity A549 cells BEAS 2B cells |
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