Premature chromatid separation and altered proliferation of human leukocytes treated with vanadium (III) oxide |
| |
Authors: | Rodrigo Anibal Mateos-Nava Juan José Rodríguez-Mercado |
| |
Affiliation: | 1. Facultad de Estudios Superiores-Zaragoza, UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UNIGEN) Laboratorio 5-PA Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Ciudad de México, México and;2. Posgrado en Ciencias Biológicas, UNAM, Edificio E, Primer Piso, Circuito de Posgrados, Ciudad Universitaria Del. Coyoacán, Ciudad de México, México;3. Facultad de Estudios Superiores-Zaragoza, UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UNIGEN) Laboratorio 5-PA Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Ciudad de México, México and |
| |
Abstract: | Vanadium is a widely distributed metal in the Earth’s surface and is released into the environment by either natural or anthropogenic causes. Vanadium (III) oxide (V2O3) is present in the environment, and many organisms are exposed to this compound; however, its effects at the cellular and genetic levels are still unknown. Therefore, in this study, the ability of V2O3 to induce chromosomal damage and impair cell proliferation was tested on human leukocytes in vitro. The cultures cells were treated for 48?h with different concentrations 2, 4, 8 or 16?μg/mL of V2O3, and we use the sister chromatid exchange’s (SCE) test and the viability assay to evaluate the effects.In the results, no change was observed in either the viability or the frequency of SCE; however, a significant increase was observed in the incidence of premature chromatid separation (PCS), and a decrease was observed in both the mitotic index (MI) and the replication index (RI). Therefore, it can be suggested that V2O3 induces a genotoxic effect at the centromere level, indicating that it is a cause of aneuploidy that is capable of altering cell cycle progression. |
| |
Keywords: | Vanadium (III) oxide lymphocytes sister chromatid exchange citostatic premature chromatid separation |
|
|