慢性肾衰竭大鼠肾移植后骨髓红细胞生成素受体的变化

目的通过观察慢性肾衰竭大鼠肾移植前后骨髓红系祖细胞的红细胞生成素受体(EPOR)的变化及与肾功能和贫血的关系,探讨红细胞生成素受体(EPOR)抑制在肾性贫血中的作用。方法以慢性肾衰竭(CRF)大鼠作为受者行同种异体肾移植术模拟人类肾移植。模型建立后RT-PCR法测定假手术组、CRF组和肾移植后不同时间大鼠骨髓红系祖细胞EPORmRNA表达的变化,WesternBlot分析EPOR蛋白质含量变化趋势,同时作EPORmRNA、EPOR蛋白质含量、Scr、Hb之间的相关性分析。结果CRF模型在5／6肾切除后90d时成功,接受肾移植后其血清肌酐和尿素氮迅速下降。CRF时EPORmRNA的表达和EPOR蛋白质含量较正常对照组明显降低,肾移植术后第7d开始二者明显升高,14d时恢复正常。相关性分析显示,EPORmRNA、EPOR蛋白含量、血红蛋白(Hb)均与血清肌酐呈显著性负相关,而EPORmRNA、EPOR蛋白含量与Hb呈显著性正相关。结论大鼠接受肾移植,尿毒症毒素完全清除后EPORmRNA及EPOR蛋白质含量显著增加至正常水平,提示EPOR抑制是引起肾性贫血的重要机理之一。

The change of erythropoietin receptor of rat bone marrow after renal transplantation

Objective To explore the important role of erythropoietin receptor (EPOR) in the mechanisms of renal anemia via observing the change of EPOR of rat bone marrow and analyzing the relations between graft function and anemia after renal transplantation. Methods The chronic renal failure(CRF) rats were surgically induced by 5/6 nephrectomy. CRF rats were recipients of renal allograft. The expression of EPOR mRNA and the content of EPOR protein of bone marrow were examined by RT-PCR technique and Western Blot analysis pre- and post-transplant. Correlations between EPOR mRNA, the content of EPOR protein, serum creatinine(Scr), and hemoglobin(Hb) were analysed. Results CRF model were successfully established on post operation days (POD) 90. The Scr and BUN decreased sharply immediately after allograft transplantation. Expression of EPOR mRNA and content of EPOR protein of CRF rats were significantly lower than normal level. After transplantation, expression of EPOR mRNA and content of EPOR protein increased significantly on POD 7 and recovered on POD 14. Negative correlations were found between the expression of EPOR mRNA or content of EPOR protein and Scr respectively, whereas statistically positive correlations were found between the expression of EPOR mRNA or content of EPOR protein and Hb. Conclusions EPOR mRNA and the content of EPOR protein recovers to normal level after allograft transplantation when uremic toxins are eliminated completely. It indicated that the decrease of EPOR of CRF rats is an important mechanism of renal anemia.