VEGF和bFGF联用对小鼠卵巢组织移植后的影响

【摘要】 目的 观察血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)对同种异体卵巢移植后卵泡的存活和血管再生的作用。方法 将18日龄ICR雌性小鼠卵巢移植到成年ICR雌鼠的颈后皮下,将小鼠分为4组,分别皮下注射10μL生理盐水、bFGF、VEGF和bFGF+VEGF,每日1次,连续注射4d,于7d后处死取材,用HE染色观察卵泡的形态和数目,CD31抗体免疫组织化学染色观察血管密度。结果 移植前新鲜的小鼠卵巢组织内各级卵泡数目多,卵泡结构形态好。与移植前相比,各组移植后的卵巢组织内一些卵泡结构出现异常,各级卵泡数目及卵泡总数显著减少。VEGF+bFGF组始基卵泡数和移植物血管密度均优于bFGF和VEGF组,差异有统计学意义。结论 VEGF和(或)bFGF干预,能够促进卵巢组织移植后血管生成,减少卵泡丢失,从而改善卵巢组织移植后的质量。

The Effects of VEGF and bFGF on Ovarian Tissue Transplantation in Mice

Objective To investigate the effects of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on the follicular survival and angiogenesis after ovarian tissue heterotopic transplantation by mouse model. Methods Ovarian tissues isolated from 18-day-old ICR outbred mice were transplanted into subcutaneous part of nuchal region of adult female mice. They were divided into four groups: A) injected with 10μl DPBS as control; B) injected with 10μl of bFGF (50μg/ml); C) injected with 10μl of VEGF (25μg/ml); D) injected with 10μl of bFGF (50μg/ml) + VEGF (25μg/ml). We injected subcutaneously around the grafted ovarian tissue once a day for four days. The grafts were retrieved 7 days after transplantation. The number and morphology of follicles were observed by H.E. staining and vascular density by IHC staining of anti-CD31 antibody. Results The number of primordial follicles in group B and group C (17.25 ± 2.55; 18.88 ± 2.42 respectively) was significantly higher than that in group A (13.25 ± 2.49) (P < 0.01), while there was no statistical difference between group B and group C; the number of primordial follicles in group D (22.50 ± 3.42) was higher than that in group B and group C (P < 0.05). Compared with the control group, the vascular density in group B and group C increased, significantly higher than group A (P <0.05); but there was no statistical difference between group B and group C; vascular density in group D were statistically higher than that in group B and group C respectively (P < 0.05). Conclusion Intervention with VEGF and / or bFGF promoted the angiogenesis after ovary tissue transplantation, while reducing the loss of follicles. That benefits improving the quality of ovarian tissue survival after transplantation.